从水稻突变体库中筛选到一个生育期延迟突变体，主要表现为生育期延迟、植株矮化、叶色变深、叶角张大、根系变短。遗传分析表明该突变体受1对隐性单基因控制。对突变体及其后代分离群体做Basta抗性检测，证实该突变体是由T-DNA插入引起的，突变性状与T-DNA共分离。PCR和Southern杂交结果进一步证实了上述观点。该材料可用于插入座位的基因克隆和生育期调控机理的研究。
Recent advances in genomic studies and the sequenced genome information have made it possible to utilize phenotypic mutants for characterizing relevant genes at the molecular level and reveal their functions. Various mutants and strains expressing phenotypic and physiological variations provide an indispensable source for functional analysis of genes. Because rice is easy to transform, T-DNA has been used successfully to generate insertional mutant lines. A large rice insertional mutant pool generated with Ac/Ds transposone system derived from maize was established in China National Rice Research Institute. A new delayed growth mutant, named T456, was screened from 45 000 independent Ds transfer lines of rice (Oryza sativa L.). It mainly performed delayed growth，dwarfism，dark green leaf color，as well as large angle of leaf to clum and short length of seminal root, but there were no differences in photosynthesis rate and respiration rate between T456 and wild-type rice cv Zhonghua 11. Genetic analysis showed that the plants with mutant phenotype and wild phenotype in T1 generation fitted to 1:3 and there were no segregation in T2 generation with mutant phenotype，indicating that the mutant phenotype was controlled by a single recessive gene. Basta determination on the healthy rice leaves in T1 and F2 generations showed that the mutant phenotype was co-segregated with the T-DNA. PCR and Southern blotting analysis further confirmed that the mutant phenotype was caused by T-DNA insertion with single copy. This insertional T-DNA mutagenesis will be useful for isolating the tagged gene in rice by Tail-PCR method easily, and for elucidating the gene functions accordingly.
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T456 Zhonghua 11 T456 T456 Zhonghua 11
图 1 中花 11和 T456成株期表型（A）和苗期根系性状（B）
Fig.1. Phenotypes of Zhonghua 11 and T456 at late developmental stage(A), and their roots at seedling stage(B)
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图 2 中花 11和T456的T1代PCR
Fig. 2. PCR results of T456 in T1 generation and Zhonghua 11
1,中花 11；2，质粒；3-20，无突变表型的 T456后代；21-30，有突变表型的 T456后代。
1, Zhanghua 11; 2, Plasmid; 3-20, Progenies of T456 with normal phenotype; 21-30, Progenies of T456 with mutant phenotype.
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图 3 中花 11和 T456的 Southern杂交
Fig.3. Southern blotting analysis of T456 mutant and Zhanghua 11
1， 中花 11（HindⅢ）；2，T456（HindⅢ）；3，T456(DraⅠ)；4，T456(EcoRⅤ)；5，T456(BamHⅠ)；6，T456(EcoRⅠ)。
1, Zhonghua 11（HindⅢ）；2，T456（HindⅢ）；3，T456(DraⅠ)；4，T456(EcoRⅤ)；5，T456(BamHⅠ)；6，T456(EcoRⅠ).