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作 者 ：杨征;蔡陈棱;何光存;覃瑞;宋运淳

期 刊 ：作物学报 1999年 25卷 06期 页码：707-711

关键词：Ca2+；细胞凋亡；水稻；愈伤组织；DNA片段化；

Keywords:Ca2+, Apoptosis, Rice, Callus, DNA fragmentation,

摘 要 ：用CaCl2处理水稻愈伤组织，研究钙离子在水稻细胞凋亡过程中的作用。结果发现，水稻愈伤组织经150mmol/LcaCI2处理1~2h后，DNA无明显降解；处理3h后，DNA降解为5kb以上的大片段；处理8h后，DNA几乎完全降解成200bp~1kb的小片段。由此推测，细胞外高浓度的Ca2+对诱导凋亡细胞的DNA片段化起了重要作用。研究还发现，一定浓度的M

Abstract:To study the function of Ca2+ in plants cell apoptosis，rice callus was treated with CaCl2 and the products were detected by agarose gel electrophoresis. The results showed that the callus’ DNA was degraded into fragments which were more than 5 kb in size after treatment with 150 mmol/L CaCl2 for 3

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