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作 者 ：章美云;韩碧文

期 刊 ：作物学报 1992年 18卷 01期 页码：17-22

关键词：烟草；薄细胞层培养；开花梯度；

Keywords:Nicotiana tabacum, Thin cell layer culture, Floral gradient,

摘 要 ：普通烟草(Nicotina tabacum L.c.v.Samsun)营养生长植株和开花植株表皮薄层在生长素和细胞分裂素配比下,可诱导花原基和成花。不同部位茎表皮薄层外植体的生化分析表明;α-淀粉酶活性向顶递增,而过氧化物酶活性及其同工酶水平向顶递减。在营养生长植株中内源游离 IAA 含量变化不显著,而在开花植株中,花梗表皮薄层游离 IAA 含

Abstract:In vitro differentiation of floral buds can be obtained from thin cell layer culture(oneto six layers of epidermal and subepidermal cells)of flower stalk sections of N.tabacum L.cv.Samsun by varying the auxin-cytokinin ratio in MS medium and cultural condition.Some biochemical analyses were conducte

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