Abstract:Transformed crown tissue cultures from Trichosanthes kirilowii Maxim. were established by Ti plasmid in C58 strains. The crown issues grew well in free- hormone MS medium . Nopaline was inspected in the crown gall and this data showed that T-DNA of Ti plasmid had been transformed successfully .The content of proteins in the tissue was about 130.6mg/g (fresh weight)which was inspected by compound-color methods and its TCN-protein was inspected by SDS-PAGE analysis. The study showed that the production of TCN proteins from crown tissue cultures may be developed in prospect.