Abstract:A acetylenic acid, ocetadecanoic acid - 9 - yne has been isolated from the stem of Diploclisia glaucescens. Its structure was elucidated by spectral method and chemical reaction. In addition, triacontanoic acid, undecanoic acid and β - sitosterol were also obtained. One of them, ocetadecanoic acid - 9 - yne was isolated from D. glaucescens for the first time. It shows stronger PAI- 1 bioactivity.