Abstract:In this paper, we analyzed the phylogenetic relationship of Thuidiaceae (s.l., Musci) using nuclear ribosomal DNA internal transcribed spacers (ITS), from which we got 6 species‘ ITS sequences of Thuidiaceae (s.l.), including Abietinella abietina (AJ417494), Anomodon minor (AJ344145), Cl-aopodium aciculum (AJ315968), Thuilium pristocdyx (AJ416443), Thuadium assimile (AJ416442) and Herpetineuron toccoae (AJ315967), and then they were sequenced directly from PCR products.