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作 者 ：刘继梅,陈善娜,杨明挚,鄙波,黄兴奇

期 刊 ：云南植物研究 1998年 20卷 02期 页码：1-3

关键词：简并引物；聚合酶链式反应(PCR)；甘油-3-磷酸转酰酶(GPAT)；

Keywords:Degenerate primer,

摘 要 ：应用高简并引物，结合PCR扩增技术从南瓜 Cucurbita moschata 总RNA的逆转录产物中得到了GPAT基因保守区。在PCR反应中，简并引物之间的浓度配比是影响扩增效率的关键。用脱氧次黄苷(dI)替代简并引物中的高简并位点，可大幅度降低简并程度，显著提高扩增产物的特异性及扩增效率。

Abstract:Using highly degenerate primers by RT-PCR, the conserved region in GPAT-gene was obtained from total RNA of squash (Cucurbita moschata). In the PCR reactions, the coneen- tration ratio between the degenerate primers was essential for influencing amplification efficiency. Utilizing deoxyinosine(dI) can decrease degenerate degree and enhance the specificity of PCR products and amplification efficiency.

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