Abstract:Floral primordia formed on the surface of the transitional region between cotyledonary blade and petiole of cucumis in 2~ 5 days on in vitro cultivation. After further development of 2~3 days, there appeared a whorl of secondary prominences on the surface of floral primordia, which indicated that the formation of sepal primordia. Supplementing with kinetin (KT) in the medium, the floral primordial formation and differentiation were promoted and the floral bud formation also improved.