Abstract:Four antitumor annonaceous acetogenins were isolated from seed of Annona glabra Linn, by column chromatography on silica gel. Three of them were identified as cherimolin-2(2), annonin 1(3) and desacetyluvaricin (4) by comparison with literature. One of them is new, with structure elucidated as 4-desoxycherimolin-2(l) by spectroscopic methods.