Abstract:This paper indicates the effects of hormone, light, temperature and time of incubation on anthocyanin contint in plantlets of Begonia fimbri-stipula Hance on uitro.NAA of concentration from 0.125 mg/l to 1.0 mg/l could increased anthocyanin content,0.125 mg/l had the best effect.BA,2ip, ZT decreased anthocyanin content. Light had a marked effects to enhance anthocyanin content of the plantlets ln the range of experiments anthocyanin content increased with the irradiation intensity. The effects of blue, white, red light used alone of conbinatly were combinatoy were compared, the effect of enhancing the