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作 者 ：许春辉 赵福洪 王可玢 储钟稀 毛大璋 戴云玲

期 刊 ：云南植物研究 1984年 6卷 04期 页码：1-3

关键词：大豆；氧活性；光系统Ⅱ颗粒；光合特性；

Keywords:characteristics, oxygen-evolving, photosystem,

摘 要 ：用去垢剂Triton X-100 从大豆叶绿体制备出具放氧活性的光系统Ⅱ颗粒。放氧活性达208 μmol O2 mg-1 Chl．hr－1，以吸氧表示的PSI活性以及低温荧光发射中代表光系统I的F735均比叶绿体明显下降。光诱导P700 吸收变化很难检测出来，用SDS一聚丙烯酰胺凝胶电泳几乎分离不出属光系统Ⅰ的叶绿素蛋白质复合物带来。代表光系统Ⅱ活性的DCIP光还原活性很高。颗粒的Cbl a/b人值为1.9。以上结果表明这颗粒十分富含光系统Ⅱ和放氧系统，其光系统Ⅰ含量是可以忽略的。在文中也叙及从菠菜和蓖麻叶绿体得到的相似颗粒的一些光合特性。

Abstract:Oxygen-eyolving PSII particles were prepared from soybean chloroplasts wiht detergent Triton X-100. The oxygen evolution activities rea-ched 208 umol O2 mg-lchl. hr-1. PSI-dependent O2 uptake and fluorescence emission peak F735 which contributed to PSI were decreased obviously. Light- induced P700 signal was hardly detected and we can‘t isolate any chlorophyll- protein complex band belonging to PSI using SDS-PAGE. The DCIP photore- duction activity was high. The Chl a/b ratio of the particles was about 1.9. The results cited above show that the soybean particles were enriched in PSlI and oxygen-evolving system and its PSI content is neglectable. Some photosynthetic characteristics of similar particles from spinaeh and castorbean chloroplasts were also showed.

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