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期 刊 ：植物遗传资源学报 2014年 15卷 2期

关键词：杨树；microRNA；MIR171基因家族；进化；功能分化；

Keywords:Populus trichocarpa, microRNA, MIR171 gene family, evolution, function diversification,

摘 要 ：MicroRN（miRNA）是一类重要的非编码小分子RNA，广泛参与植物生长发育和胁迫响应的调控。杨树MIR171基因家族是一个古老的miRNA基因家族，具有14个成员。本文对杨树MIR171基因家族的基因倍增模式、表达方式、启动子结构及靶基因进行了分析。结果表明：杨树MIR171基因家族主要通过48-54百万年前的染色体大片段重复进行扩张，其表达方式和功能已经出现分化。MIR171基因家族可能主要通过调控GRAS转录因子和信号转导蛋白参与杨树生长发育、光信号转导和光形态建成的调控。

Abstract:MMicroRNAs (miRNA) are a class of non-coding small RNAs that function as regulators of development and stress responses in plants. The ptc-MIR171 gene family is an old gene family which consists of 14 members. In this paper, the duplication, expression patterns, promoters and target genes of poplar MIR171 gene family are analyzed. The results showed that poplar MIR171 gene family expanded mainly through large-scale duplication 48-54 million years ago (MYA) and the expression patterns and functions have diversified. Poplar MIR171 gene family involves in complex regulation of development, light responsiveness and photomorphogenesis by targeting GRAS transcription factors and signal transduction proteins.

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