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期 刊 ：植物遗传资源学报 2016年 17卷 5期

关键词：茶树；钙调素CaM；低温胁迫；表达分析；

Keywords:tea plant, CaM, cold stress, expression analysis,

摘 要 ：摘要：钙调素（CaM）是植物钙离子信号通道的主要参与者，参与低温胁迫下多种植物的抗寒生理作用。本研究根据钙调素基因相关表达序列标签（EST）序列，借助RACE-PCR技术，获得CsCaM1 和 CsCaM2两条cDNA 全长序列，GenBank 登录号分别为KT238971和KT238972，长度分别为693 bp和841 bp，均包含450 bp 的完整开放阅读框（ORF），编码149个氨基酸，两条氨基酸序列仅有一个氨基酸有差异，且均含有四个植物CaM家族的共同特征手型结构EF（EF-hand）。采用实时荧光定量 PCR（qRT-PCR）分析CsCaMs在茶树低温胁迫下各种处理中的表达模式。结果表明, CsCaMs无组织表达特异性，低温胁迫处理和CaCl2均能诱导CsCaMs的表达，而钙调素拮抗剂W7与钙离子通道抑制剂LaCl3则会抑制其表达。本研究结果对阐明茶树抗寒性的分子机理有一定理论意义，为茶树的抗寒性育种提供了新的思路。

Abstract:Abstract: Calmodulin (CaM) is a major participant in calcium signaling pathway, involved in multiple physiological processes of plant under cold stress. Using RACE-PCR and RT-PCR, two full-length cDNAs of CsCaM were cloned from tea plant based on the available EST sequences of calmodulin gene. CsCaM1 had 693 bp in length, and CsCaM2 had 841 bp in length. Both of them contained a 450bp open reading frame (ORF) encoded 149 amino acid residues. CsCaMs had four EF-hands, which are CaM gene familes’ characteristic. CsCaM1 and CsCaM2 were submitted to GenBank with accession number KT238971 and KT238972, respectively. The expression profiles of CsCaMs under cold treatment were investigated using qRT-PCR (quantitative real-time PCR). Results indicated that the expressions of CsCaMs were induced gradually with the extension of cold treatment. Under CaCl2 treatment, the expressions of CsCaMs were up-regulated. However, the expressions of CsCaMs were down-regulated under W7 and LaCl3 treatments. Above results demonstrated that CsCaMs might play an important role in cold tolerance of tea plant.

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