摘 要 ：为研究无核小枣果实无核形成机理，应用PCR扩增技术结合基因克隆技术从无核小枣果实中得到4-香豆酸辅酶A连接酶(4-Coumarate:Coenzyme A Ligase,4CL)基因的完整编码区，序列长度1819bp，包含1776bp的完整开放阅读框，编码591个氨基酸，命名为Zj4CL(登陆号KP279930)。Zj4CL与基因组序列比对分析发现，该基因组含有5个外显子，4个内含子。其序列比对和结构域分析表明，无核小枣的4CL基因包含4CL 基因家族的 Box I和Box II保守区，进化树分析显示Zj4CL与苹果（XP_008376904）的亲缘关系最近。实时荧光定量PCR分析表明，无核小枣在花后30d左右基因表达量达到最大值，到花后40d以后表达量明显下降。
Abstract:To study of forming mechanism of fruit stoneless, 4CL genes in full length with 1819bp coding sequence encoding protein of 591 amino acids was obtained from Ziziphus jujuba Mill.‘Wuhexiaozao’ by the PCR technology. It is named as Zj4CL (accession number KP279930).The alignment of Zj4CLand genomic sequence found that,the genome contains 5 exons and 4 introns.Sequence alignment and motif analysis showed that Zj4CLgene included Box I and Box II of 4CL gene families. Protein sequence alignment showed it had a highest genetic relationship with Malus domestica (XP_008362825). The results of qRT-PCR revealed that the highest expression levels were appeared on the 30th day after flowering. The expression 40th day after flowering declined apparently.