Estimating copy number of transgenic gene by real-time fluorescent quantitative PCR(TaqMan) Development and Application of Real-time Fluorescent Quantitative PCR for Detection of Pseudomonas tolaasii Effects of Bagging Treatment on Sugar Metabolism and Related Gene Expression in‘Chili’Pear Fruits Effects of Bagging Treatment on Sugar Metabolism and Related Gene Expression in‘Chili’Pear Fruits Cloning and Expression Analysis of AP1-like Gene from Sedirea japonica Molecular Cloning、Correlation Analysis and expression studies of 4CL Gene in Ziziphus jujuba Mill.‘Wuhexiaozao’ Effects of vegetable cultivation years on microbial biodiversity and abundance of nitrogen cycling in greenhouse soils. Detection of Trichoderma asperellum colonization in soils by real-time fluorescent quantitative PCR