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期 刊 ：植物遗传资源学报 2014年 15卷 2期

关键词：中国；茶树；种质资源；生物碱；鉴定；特异资源；

Keywords:China, tea plant, germplasm, purine alkaloid, identification, rare germplasm,

摘 要 ：从国家种质杭州茶树圃选取了403份代表性的茶树资源，采用HPLC对嘌呤生物碱进行了两年、春秋两季的重复鉴定。结果表明，年度和季节间咖啡碱含量总体稳定，但可可碱含量春季变化显著。93%以上资源的咖啡碱含量为25.0 ~ 45.0 mg/g。茶的3个变种间，咖啡碱含量存在着显著差异，且阿萨姆茶秋季较春季咖啡碱含量明显增加。来源不同地区的资源咖啡碱含量存在着显著差异，其中云南和广东的资源变异系数和多样性指数最大。从403份资源中筛选出3份高咖啡碱、1份高苦茶碱（低咖啡碱）和2份高可可碱（低咖啡碱）等6份特异资源

Abstract:A set of 403 accessions tea genetic resources were selected from China National Germplasm Hangzhou Tea Repository (CNGHTR) to test the purine alkaloids content of spring and autumn in two years using HPLC. It revealed the caffeine content of tea genetic resources were relatively stable between years or seasons, while the theobromine content had a significant variation in spring. The caffeine content of more than 93% resources varied from 25.0 to 45.0 mg/g. Caffeine content had a significant difference in the three varieties of Camellia sinensis (L.) O. Kuntze, and that of assamica variety in autumn was significantly higher than the spring’s. The variation of caffeine among different provinces had been investigated. It had a significant difference in the contents, and Guangdong and Yunnan had higher coefficient of variation (CV) and diversity indices (H’). Moreover, 6 specific tea accessions were screened out, including 3 high caffeine, 1 low caffeine and high theacrine, 2 low caffeine and high theobromine accessions, respectively.

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