摘 要 ：以源于二倍体梨品种 ‘Fertility’ (Pyrus communis L.)通过秋水仙碱离体诱变体细胞染色体加倍获得的不同同源多倍体无性系为试材，以离体叶片为外植体，观察研究了不同倍性无性系叶片的不定梢再生能力。结果表明，多倍体的不定梢再生率显著低于二倍体的再生率。不同多倍体无性系的不定梢再生能力也存在显著差异。三倍体无性系‘3x-3’和四倍体无性系‘4x-4’不能诱导产生不定梢。表明器官发生能力下降或植物细胞全能性的丧失与细胞染色体多倍体化有关。
Abstract:Shoot regeneration ability from in vitro leaf explants of different ploidy clones which were derived from the same diploid cultivar of pear (Pyrus communis L.) by colchicine treatment was examined. The regeneration rates of neopolyploids were significantly lower than that of diploid control. Regeneration rates among different neopolyploid clones were significantly different. Triploid ‘3x-3’ and tetraploid ‘4x-4’ failed to regenerate. These results revealed that the decrease of regeneration ability or loss of totipotency was related to the polyploidization.