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期 刊 ：植物遗传资源学报 2014年 15卷 3期

关键词：设施黄瓜；品种；芽期耐盐；隶属函数；

Keywords:reenhouse cucumber, Cultivars, Slat tolerance at germination stage, Subordinate function,

摘 要 ：用100 mmol/L Mg(NO3)2盐溶液对不同生态类型的143个设施黄瓜新品种进行4天芽期胁迫处理，调查各种质的发芽势、发芽指数、相对伤害率、发芽率、相对发芽率、第2天和第4天胚根伸长长度、后2天胚根伸长速度等8个指标，利用各指标隶属函数值对其耐盐性进行聚类分析，并对耐盐强的种质进行恢复出苗初步筛选。研究结果表明，黄瓜萌发期的耐盐性强弱评价结果受多个指标的影响。8个发芽指标的变异系数为10.5%-81.0%，除了发芽率和相对发芽率的变异较小外，其他性状的变异幅度均较大（32.0-81.0%）；各性状指标变异系数在不同生态类型种质中表现不同，华北型和欧洲温室型种质以盐害指数变异系数为最大，华南型和欧美加工型种质以后2天胚根伸长速度变异系数最大。从种质总体抗盐结果看，欧美加工型和欧洲温室型>华南型>华北型。各指标隶属函数值聚类分析结果表明，将所有种质划为耐盐强、中、弱3类，其中耐盐强的品种共有54个，占37.8%。54个耐盐品种胁迫后经出苗试验初步筛选出12个恢复能力强的品种，即：J31-1＞绿精灵＞DRT5＞YT24＞东农02号＞上海农科4号＞冬灵102＞IVF8＞津优35＞HGS-2＞扬大13＞J30-1。

Abstract:143 newly-bred greenhouse cucumber cultivars belonging to different ecotypes were treated with 100mM solution of Mg(NO3)2 for 4 days at germination stage. And eight indices including germination potential, germination index, salt-damage index, germination frequency, relative germination frequency, radicle length of 2 and 4 days after salt treatment, radicle elongation speed of the latter 2 days were investigated. Using the Membership function value of these indices to cluster the new-bred greenhouse cucumber cultivars, and primarily select salt-tolerance germplasms through growing them under salt-free conditions after 4 days salt treatment. The results showed that cucumber salt-tolerance at germination stage were affected by multiple factors. The variation coefficient of eight indices were ranged as 10.0%-81.0%. Except variation coefficient of germination frequency and relative germination frequency was less, other indices variation coefficient was larger as 32.0-81.0%. The variation coefficient of these indices was different among different ecotype germplasm. The variation coefficient of salt-damage index is the largest of North-China Ecotype and European Greenhouse Ecotype, however in South-China Ecotype and European-processing type germplasms, the variation coefficient of radicle elongation speed of the latter 2 days is the largest. From overall view of Germplasm Salt tolerance, European greenhouse Ecotype and Processing Ecotype is better than South-China Ecotype, and then South-China Ecotype is better than North-China Ecotype. All the germplasm were divided into three groups according to the cluster results of Subordinate function value: better, moderate, and less salt-tolerance. There are 54 high salt-tolerance cultivars as accounting for 37.8%. By emergence selection of these high salt-tolerance germplasm after salt treatment, 12 cultivars were chosen as better restore-growth germplasm. They were arranged as J31-1＞Lvjingling＞DRT5＞YT24＞dongnong2> Shanghanongke 4＞Dongling 1 02＞IVF8＞Jinyou35＞HGS-2＞Yangda13＞J30-1 according to their restore-growth ability.

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