Abstract:The sterile multiplied shoots of the adult Crataegus scabrifolia (Franch) Rehd. were used as the experiment material of rooting. The results sho- wed. shoots inoculated to culture mcdium of MS/ 2 added with auxin (IBA, NAA) at 0.01-1 .0mg/l, the rooting percentage was 50.4%. When the bases of the shoots first were immersed in high auxin concertration (l50--250mg/l) solution for 30 minutes, then inoculated to the culture medium of MS/ 2 devoid of auxin the rooting percentage was 60.1%; if dipping the bases of shoots for 1-2 seconds, the rooting percentage was 83.5%3 if the shoots were cultured for 2- 4 days in MS /2 medium with IBA at 1-5mg/l, then were transfered to the same medium but devoid of auxin, 15 days or so, the rooting percentage was over 92% and roots elongated normal; the rooting almost was not influenced with temperture between 20-29℃; rooting was obviously inhibited in the dark, unsatisfied under light condition 8 hours a day, but very satisfied in 16 hours or 24 hours a day.