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期 刊 ：植物遗传资源学报 2016年 17卷 1期

关键词：辣椒；种质资源；真核转录因子(eIF4E)；多态性；

Keywords:Capsicum annuum, germplasm resources, eukaryotic translation initiation 4E, polymorphism,

摘 要 ：利用国家蔬菜种质资源库 辣椒资源1904份材料，采用测序技术获得eIF4E（eukaryotic translation initiation 4E）基因exon1序列，研究eIF4E基因多样性及我国辣椒种质资源群体多样性。结果表明：在1904份材料中共发现17个单倍型，14个有义多态性位点，其中9个为新的位点，位点大多集中在eIF4E蛋白表面环上； 8个地理群体的平均单倍型多样性（Hd）和平均核苷酸多样性（Pi）分别为0.519和0.00210；群体间分化指数（Fst值）及基因流（Nm）表明不同群体间表现为差异的分化程度；AMOVA分析表明总变异主要来源于群体内个体间的变异（97.23%），只有2.77%变异发生在群体间。本研究将有助于了解我国辣椒eIF4E基因多样性，为抗PVY育种挖掘更多抗源材料。

Abstract:Exon 1 of eIF4E gene (eukaryotic translation initiation 4E) of 1904 pepper accessions from National Vegetable Germplasm Bank in China was sequenced and researched on the diversity . The results showed: 17 haplotypes were found among the 1904 accessions and 9 new sites in the 14 sense polymorphic loci which mostly concentrated on the eIF4E protein surface ring; the average haplotype diversity(Hd) and average nucleotide diversity(Pi) of 8geographic populations were 0.519 and 0.00210; differentiation index(Fst value) and gene flow(Nm) between populations showed that it performs different degree of differentiation among groups; AMOVA analysis showed that the total variation comes mainly from individuals within populations(97.23%), only 2.77% of the variation occurred between populations. This research is helpful to understand the diversity of the eIF4E gene, providing more resistant resources for pepper PVY resistance breeding.

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