摘 要 ：Dof (DNA-binding with one finger)转录因子是植物特有的一类转录因子，该类转录因子在植物生长发育过程中起重要作用。本实验以胡萝卜‘黑田五寸’和‘君川红’为实验材料，分别从中克隆得到DcDofD1转录因子基因。采用生物信息学方法对DcDofD1转录因子氨基酸组成、理化性质、进化关系进行了分析。并利用实时定量PCR方法对DcDofD1基因在非生物胁迫下的表达量进行了分析。结果表明‘黑田五寸’和‘君川红’中的DcDofD1转录因子具有明显的单锌指结构，保守域的氨基酸序列比较保守，进化分析显示，DcDofD1属于Dof家族的D1亚族。在胡萝卜‘黑田五寸’和‘君川红’中，DcDofD1基因对高温、低温、干旱、盐等非生物胁迫响应。而不同胡萝卜材料中，DcDofD1基因对非生物逆境响应的强度和速度不同。
Abstract:In higher plant, Dof (DNA-binding with one finger) transcription factor is a plant-specific transcription factor, which plays important roles during the growth and development. Here, the genes of DcDofD1, which encoding to a Dof transcription factor, were cloned from two carrot varieties ‘Heitianwucun’ and ‘Junchuanhong’, respectively. The sequences of nucleic acid and amino acid, phylogenetic tree, hydrophilicity and hydrophobicity, and molecular modeling were analyzed using bioinformatics method. The results demostrated that the two DcDofD1 factors from carrot had single zinc finger, and their amino acid sequence at Dof domain were very conserved. The DcDofD1 factors from carrot were classified into D1 subfamily of Dof family. The expression profiles of the DcDofD1 gene under four abiotic stress treatments (cold, heat, high salt, and drought) were analyzed by quantitative real-time PCR in the two carrot varieties ‘Heitianwucun’ and ‘Junchuanhong’. The results of expression profiles showed that DcDofD1 gene responded to the cold, heat, high salt, and drought stresses in carrot. The results demostrated that the DcDofD1 gene involved into interact with abiotic stress in carrot.