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期 刊 ：植物遗传资源学报 2014年 15卷 6期

关键词：ANK重复序列；生物信息学；亚细胞定位；表达模式；菜豆；

Keywords:Ankyrin repeats, bioinformatics, subcellular localization, expression pattern, Snap Bean,

摘 要 ：锚蛋白重复序列模体是生物体内最普遍的蛋白质序列模体之一，在多种细胞活动中主要介导蛋白质-蛋白质的相互作用。利用菜豆基因组数据库，通过生物信息学手段对菜豆ANK家族成员及分子生物学特性鉴定。菜豆基因组中含有30个ANK家族基因，分布于9条染色体上，其中第五条染色体上含有ANK的基因最多，包含13个基因。蛋白结构域分析发现ANK25除了含有ANK结构域外还含有RING结构域。据报道，RING蛋白主要参与基因转录调控、DNA修复和重组等。亚细胞定位结果显示，ANK25主要分布在细胞膜上。表达模式分析发现，ANK25对干旱、盐和ABA胁迫有响应。本研究为进一步研究菜豆ANK的分类及功能提供了有利的依据。

Abstract:Ankyrin repeats, as one of the most commonly protein motifs, are involved in diverse protein-protein interactions in various life activities. Based on Snap Bean genome database and bioinformatics method, we obtained Snap Bean ANK family genes and analyzed their molecular biological characteristics. A total of 30 ANK genes were systematically identified from Snap Bean and located on 9 chromosomes. The chromosome 5 has 13 genes, which is more than other chromosomes. Protein structure domain analysis showed that ANK25 still contains a RING domain besides an ANK domain. Ring proteins are mainly involved in gene transcriptional regulation, DNA repair and recombination. The transient expression in Arabidopsis protoplasts displayed that ANK protein mainly contributed in plasma membrane. Expression pattern analysis found that ANK25 is responsive to drought, salt and exogenous ABA. This study provides valuable information regarding the classification and functions of ANK genes in Snap Bean.

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