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Target protein candidates of hypothalamus in aging rats with intervention by Qiongyugao

琼玉膏延缓衰老作用的候选靶蛋白分析



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Targetproteincandidatesofhypothalamusinagingratswith
interventionbyQiongyugao
QUWeiling,CAOYuan,LIUHuanlian,TONGLing,FANRui,LIURongfu
(GuangzhouUniversityofChineseMedicine,Guangzhou510006,China)
[Abstract] ToexploretheassociatedproteinsofthehypothalamusinagingratmodelswithinterventionbyQiongyugao(QYG)based
oniTRAQtechnology,findoutthetargetproteincandidatesandinvestigatethemechanismofdelayingagingforQiongyugaoThere
sultsshowedthatQiongyugaoincreasedGSHPxactivityinserumandSODactivityinliver;thetotalproteincountidentifiedbyiTRAQ
was3522,FDR<1%Therewere20kindsofdiferentialproteinsbetweentheblankgroupandmodelgroup;therewere295kindsof
diferentialproteinsbetweenmodelgroupandQYGgroup,and40kindsofthemhadadiferencemultiple≥130(themaximumvalue
was147)Comparedwithblankgroup,therewere14kindsofproteinsthatweredownregulatedinmodelgroupandupregulatedin
QYGgroupCombinedwithliteraturesearchandgenefunctionsearch,12kindsoftargetproteincandidateswerescreenedout:ST18,
Ptprc,PSMB8,INPP4B,Shc3,Pik3r1,PIP5K1C,Nampt,Rasgrp2,Asah2,Pdpk1,andMap2k7Theexpressionofnuclearfactor
kappaB(NFκB)inthehypothalamicinflammatorypathwaywasdetectedbyWesternblotandtheresultsshowedthatitsexpression
levelinmodelgroup(096)washigherthanthatincontrolgroup(085),whileitsexpressionlevelinQYGgroup(089)waslower
thanthatinmodelgroupQPCRresultsshowedthattherelativemRNAexpressionlevelsofPIP5K1CandPtprcinmodelgroupwere
significantlylowerthanthoseinblankgroup(P<001);whilecomparedwiththemodelgroup,themRNAexpressionlevelsof
PIP5K1CandPtprcinQYGgroupweresignificantlyincreased(P<001)ThisresultwasconsistentwithproteomicsdataQYGmay
delayagingbyregulatinghypothalamicinflammatoryreaction
[Keywords] Qiongyugao(QYG);antiaging;targetproteincandidates;hypothalamus;iTRAQ;NFκB
doi:10.4268/cjcmm20160724
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