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Mitogen-activated protein kinase genes of Artemisia annua and their expression analysis under Cadmium stress

黄花蒿促分裂原活化蛋白激酶基因及其在重金属镉胁迫下表达分析



全 文 :2016
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MAPK)
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,Tel:(010)64011944,Email:glp01@126com
MitogenactivatedproteinkinasegenesofArtemisiaannuaand
theirexpressionanalysisunderCadmiumstress
ZHOULiangyun,LVChaogeng,KANGChuanzhi,WANGSheng,TANGJinfu,KANGLiping,GUOLanping
(StateKeyLaboratoryBreedingBaseofDaodiHerbs,NationalResourceCenterforChineseMateriaMedica,
ChinaAcademyofChineseMedicalSciences,Beijing100700,China)
[Abstract] InordertostudyArtemisiaannuaundercadmiumstress,whethertherearecorespondingMAPKgenesinvolvedintrans
ductionofthecadmiumsignal17AaMAPKgenes,namedAaMAPK1AaMAPK17repectively,werefinalyobtainedbyusingTrinity
methodfordenovoassemblyoftranscriptsfromSRAdatabaseandBLASTsearchagainstAtMAPKgenesanddetermingconserveddo
mainusingaseriesofbioinformaticstoolsThereexist16MAPKgenescontainedT[D/E]Yconserveddomainsamongtheobtained
genesTheexpressionsofthesegeneswereanalyzedbyRealtimePCRundercadmiumstressTheresultsshowedthattheexpressions
levelofAaMAPK3andAaMAPK10weredownregulatedandMAPK7,MAPK9andMAPK12wereupregulatedTheseindicatedthat
thereexistcorespondingMAPKgenesinvolvedintransductionofthecadmiumsignal
[Keywords]  Artemisiaannua;MAPKgene;Cadmiumstress;expressionanalysis
doi:104268/cjcmm20160607
  
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1 qRTPCR
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Table1 TheprimersofAaMAPKsusedinqRTPCR
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(5′3′)
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/bp

MAPK1
bO
:AAGTTCATCCTGCTGCCATTG
ÛO
:CCTTCATCTGTTCCTCGGTTG
192 TEY
MAPK2
bO
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ÛO
:CTTCAACTGTGATTCGTTTGG
109 TEY
MAPK3
bO
:TGGAGATCGCTTTGAGGGA
ÛO
:CGGGGGACACATTAGGGAA
198 TEY
MAPK4
bO
:TCATGCACCGAAGAACATATC
ÛO
:CATCACCTACACACACATACC
96 TEY
MAPK5
bO
:GAGGAGATGTTGAGGGAAATG
ÛO
:CGAGCCTTGGCTATCTTAAC
82 TEY
MAPK6
bO
:GTACCCGTGGATTTGGATATAG
ÛO
:GCAACAGCTTCAGGATGATA
98 TEY
MAPK7
bO
:GGCAACTCCAGTAGAACCACC
ÛO
:GCAAACGATCCCATAGGCAC
139 TEY
MAPK8
bO
:CAAAGAGGATTAGCGTGACAGAA
ÛO
:GGCAGTAGCAAGAACTTCAGGAT
188 TEY
MAPK9
bO
:GAAAGGTGTTCCGGTGTATG
ÛO
:CGGCCAACAGGTTGAATAG
105 TEY
MAPK10
bO
:AATGCTTTTGATAACCGTATTGATG
ÛO
:GTGCTTGAGACGACTTGATAATCTG
196 TEY
MAPK11
bO
:CAAGTTCCTCAAAGCGTCCA
ÛO
:CCTTCGTTGTTCAAGAGCCG
102 TDY
MAPK12
bO
:CAACAGGAACCCATATTACAACC
ÛO
:GGAAGAAGAGCCTCAAGCAAA
196 TDY
MAPK13
bO
:ACACCCAAATATGTAGGAGCCA
ÛO
:TTGAAGCCTTGATACCCGAAT
128 TDY
MAPK14
bO
:CCATCAAGGCGGGAATTTA
ÛO
:TGCTCTTGTGTCAAGTCATC
101 TDY
MAPK15
bO
:CCTCAACGACCGTCTATCTATTCC
ÛO
:GCTATTGTTACTTCCTGATTCTATGGC
135 TDY
MAPK16
bO
:CACGGAGTATGGAGAAGCGAGT
ÛO
:CGGATGCCTAAGCAAACGAA
199 TDY
MAPK17
bO
:GGGCACCAGAACTCATTTTTG
ÛO
:AATAGTGGCTGTCCCAGAAGG
103 —
33 AaMAPKs
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17
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。16
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AaMAPKs
Ô
Õ²­ÜÝöq
1。
7qœFGI”Osƒ

,AaMAPK3,AaMAPK102
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ƒÜÝöl¦°
20,80μmol·L-1I”•,ù{
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。AaMAPK3,AaMAPK10
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d ­ Ô Õ
。AaMAPK7,AaMAPK9,
AaMAPK12
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20,80μmol·L-1I”•$ƒÜÝö
:°­\•9I”Ëš·ÜÝö·

ù{
Ø­ËÌÍ$
AaMAPK7,AaMAPK9,AaMAPK12
ƒ
ÜÝÜë$Ηhi

“$
AaMAPK7,AaMAPK9
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ËÌÍΑÏЕ_Ñ ÒÓÔՒ“²Ö׉ØÙÚÛÜݑ›
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‰°
TDY


FG
I”Osƒ¯_
,AaMAPK6
ÔÕ²­\•¨xI
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9²­°
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AaMAPK6
ÔՃÜݜÒ
†fBô

ù{f²[vûšbÒ
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ƒÜÝ

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AaMAPK6
ÔՉ°
TEY


q
1 AaMAPKs
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Fig1 ThediferentialexpressionanalysisofAaMAPKs
4 
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
[10],
ªðB
17
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[11],
¬QB
19
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ña
17
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16
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[14]。
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,12
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[15]。
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yg?z

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$%,m

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ª:Ù

ËóŒ

–Å@

ö

×Ø

QÍõâ$Ø
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ô
âýd’
Cd
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[J].
$%$
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