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Attenuation mechanism of gamboges during processing based on inflammatory toxicity and AQP3, AQP4 protein and mRNA expressions in rat gastric and duodenal tissues

基于致炎毒性,胃和十二指肠AQP3,AQP4表达的藤黄炮制减毒机制研究



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Attenuationmechanismofgambogesduringprocessingbasedon
inflammatorytoxicityandAQP3,AQP4proteinandmRNA
expressionsinratgastricandduodenaltissues
ZHAOQin1,2,FANGYun1,PANLingyun1,XUMin1,WANGHaiying1,XIUYanfeng1
(1.SchoolofPharmacy,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai201203,China;
2.ShanghaiFengxianDistrictHospitalofTraditionalChineseMedicine,Shanghai201499,China)
[Abstract] Toexpandtheclinicalapplicationofgamboges,itisnecessarytostudycrudegamboges′toxicityafteroraladministration
andatenuationmechanismduringprocessingInthisstudy,crudegamboges′toxicitywasjudgedbymultipleassays,includinginflam
matorymediums[suchasnitricoxide(NO),tumornecrosisfactoralpha(TNFα),andinterleukin6(IL6)]releasedbymacro
phageRAW2647,andpathologicalmanifestationsofratstomachandduodenaltissuesafteroraladministrationwithcrudeandpro
cessedgambogesTheatenuationmechanismduringprocessingwasstudiedbydetectingAQP3,AQP4proteinandmRNAexpression
inratgastricandduodenaltissuesusingimmunohistochemicalassayandrealtimefluorescentquantitativePCRtechniqueAccordingto
theresults,crudegambogesgroupshowedpromotioninreleaseofNO,TNFαandIL6bymacrophageRAW2647inadosedepend
entmanner;Comparedwithcrudegambogesgroup,processedgambogesgroupshowedreductioninreleaseofNOandIL6,within
creaseinTNFαCrudegambogescouldcauseratdiarhea,whitebloodcelsincrease,lymphocytesdecrease,hyperemiaandedema
inratgastricmucosa,duodenalmucosalnecrosisandinflammatorycelsinfiltrationAloftheseresultsprovedthatgambogeshadthe
inflammatorytoxicityingastricandduodenaltissuesafteroraladministrationinadosedependentmanner,whichhoweverreducedafter
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Vol41,No.9 May,2016
processingInadditiontotheinflammatorytoxicity,themRNAandproteinexpressionsofaquaporin3(AQP3),aquaporin4(AQP4)
ingastricandduodenaltissuesofhighdosecrudegambogesgroupwereincreasedsignificantly(P<005),whiletheproteinandmR
NAexpressionsofAQP3,AQP4wereweakenedinprocessedgambogesgroupTheresultsshowedthatAQP3,AQP4proteinandmR
NAexpressionswerepositivelycorelatedwiththeinflammatorytoxicityInconclusion,downregulationofAQP3,AQP4proteinand
mRNAexpressionsmaybeoneofatenuationmechanismsinprocessinggamboges
[Keywords] gamboges;processing;toxicity;inflammation;aquaporin3(AQP3);aquaporin4(AQP4)
doi:10.4268/cjcmm20160910
  
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·9261·
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Vol41,No.9 May,2016
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