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作 者 ：王洪玲,陈浩,耿长安,张雪梅,马云保,江志勇,陈纪军

期 刊 ：中国中药杂志 2011年 36卷 11期 页码：1454.

关键词：椭圆叶花锚；蒽酮；裂环烯醚萜苷；

Keywords:Halenia elliptica, xanthones, iridoid glycosides,

摘 要 ：目的: 对龙胆科Gentianaceae花锚属植物椭圆叶花锚Halenia elliptica的干燥全草进行化学成分研究。 方法 :椭圆叶花锚的干燥全草用90% 乙醇提取,依次用石油醚、乙酸乙酯、正丁醇萃取,对乙酸乙酯萃取部分采用各种柱色谱进行分离纯化,通过波谱数据分析(¹H-NMR,¹³C-NMR,MS) 进行结构鉴定。 结果 :从乙酸乙酯萃取部分分离鉴定了12个化合物,其中5个蒽酮,1个裂环烯醚萜苷,以及6个其他类化合物。分别鉴定为8-羟基-2-甲基色原酮(8-hydroxy-2-methylchromone, 1 ),5-羟基-2-甲基色原酮(5-methoxy-2-methylchromone, 2 ),7-表断马钱子苷半缩醛内酯(7-epi-vogeloside, 3 ),松柏醛(coniferl aldehyde, 4 ),芥子醛(sinapaldehyde, 5 ),norbellidifolin( 6 ),1-羟基-2,3,4,6-四甲氧基蒽酮(1-hydroxyl-2,3,4,6- tetramethoxyxanthone, 7 ),1-羟基-2,3,4,7-四甲氧基蒽酮(1-hydroxyl-2,3,4,7- tetramethoxyxanthone, 8 ),1-羟基-2,3,5-三甲氧基蒽酮(1-hydroxyl-2,3,5- trimethoxyxanthone, 9 ),壬二酸(azelaic acid, 10 ),β-谷甾醇(β-sitosterol, 11 ),齐墩果酸(oleanolic acid, 12 )。 结论 :其中化合物 1,2 为新的天然产物,化合物 3~6,10 为首次从椭圆叶花锚中分离得到,化合物 6 显示较好的抗HBsAg和HBeAg活性,其IC₅₀分别为0.77 mmol·L^-1和<0.62 mmol·L^-1。

Abstract:Objective: To study the chemical constituents of Halenia elliptica. Method: The air-dried whole plants of Halenia elliptica were extracted with 90% EtOH. The EtOH extract was condensed to a small amount of volume and extracted with petroleum ether, EtOAc and n-BuOH, successively. The compounds were isolated and purified by column chromatography from the EtOAc fraction, and identified based on spectral analyses(MS, ¹H-NMR, ¹³C-NMR). Result: 12 compounds were isolated from H. elliptica, and characterized as 8-hydroxy-2-methylchromone( 1 ), 5-methoxy-2-methylchromone( 2 ), 7-epi-vogeloside( 3 ), coniferl aldehyde( 4 ), sinapaldehyde( 5 ), norbellidifolin( 6 ), 1- hydroxyl-2,3,4,6-tetramethoxyxanthone( 7 ), 1-hydroxyl-2,3,4,7- tetramethoxyxanthone( 8 ), 1-hydroxyl-2,3,5-trimethoxyxanthone( 9 ), together with azelaic acid , β-sitosterol, and oleanolic acid. Cloclusion: Compounds 1, 2 were new natural compounds and compounds 3-6, 10 were obtained from H. elliptica for the first time and compound 6 showed inhibitory activities against HBsAg and HBeAg secretion with IC₅₀ value of 0. 77 and<0. 62 mmol·L^-1, respectively.

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