免费文献传递   相关文献

Effects of PEG stress on flavonoids accumulation and related gene expression in suspension of Scutellaria baicalensis

PEG胁迫对黄芩黄酮类有效成分积累及相关基因表达的影响



全 文 :#stuvn
#
8-?
:;A<=<>¨èéÚ6U?G
j‰s@¼k[AB
&b´
#
cd
#
\e
!
#
f*å
#
7g
#

!
8;8HI¯J 8KLMN
#
ýþ
PMM[MM
$
†;
%!

&
ƒ„YZ.
8-?
:;A<=/0¨©r<=¤
#
<=—[U?Gj‰s@¼k[AB

ˆ‰
.
“›
.8B
”1%<=/0¨©r<=¤
#
<=—[Z·
!
›2Àß1%¨©~3456@Z·
!
>›7Ô·
3@;83
[…”Aj
‰s@[¼k8É6‹

Š‹uŠŒ
.
<=/0¨©~3456@Z·9
8-?
:;<3—f3—
!PMg8-?
:;<=/0
¨©*ü[Ç:;q
!
µ—/0¨©
#$
s@¼k·<ä=8<=—[U?
WMg8-?
:;µ—>
%&#(
s@[¼k
·
!
ìZ7
=8+
2Y›[µ—?Ü><=—ió@Y›c
!
·A><=—†<=¤[K
$
Ž
%!
<=
&
/0¨©
&8-?&
7Ô·
3@;83&
s@¼k
&
<=¤
&
<=—
$
8$f
%!WMPPMOWXMMO
$
‘’Eq
%!
­æö÷iu«u
$WMM_d+M_OMP;MML;MO%&
øùSö
÷ŠB.Cuv
$WMMN7W%&
r­DE¾ö]st¯F™Guv
$WMPMMOWWN%
$
“„>”
%!
!
HI
!@(&.$MPM%X^MP^^PP;W_LL!-;I$&.GIVOPL(V(
\PXOhG/I
!!
<=êJ|ö<=KÉÌ<=
)*+!,-./0. 1.2
0*.-,3404?(/%*
[ÇLê
!
Mò­N›uOÇÁ
!
w
èóPQR
#
LSTU
#
VQWX[E›
P(


ƒ„¼.
!
<=r‚&Y›Ú6<=¤
#
<=—
#
ø
<=¤
#
ø<=—‡<>\ÄÌ
!
wèQP
#
?N
#
Y
q
#
ZM
#
ZK62‡E›

”÷;<=¤ê‚&
ÈÂ[[#

#
]^žGë<]^ž‡j
_!ä
!
AÍ<=µ#̛·`÷ªa

<=—GªãÌÄÚ´µ‰Š2s@[¼ka
b®c*×[AB

ªr
!
„…6@Q62
$8=B%#
C>>Ä2
$.1%#
<=—
[;5;
deUf@sK^
2
$,7?=@%
Ñ
;
dUf@2
$?,1%
MÑ<>\Ä
Ì|³E›[³˜‰Š2
W;O(
!
]¡c\gQRôh
rfii&E›

H’j‡
^(
ƒ„¼.I“A
<=/0¨©rèéÚ6[U?èu¡[=8E
›
!
Z7I¶·kÄ>
#$!%&#(
[¼k

Z7
!
SlZm·`MAB<=èéÚ6[U
?[i&@—g|
!
n[[‡
L(
RoSl…m
ä=8<=pqr5<>Ñ<=—Z·[U?

¤
§¨<=/0¨©êAZ
!
m¡›YZ<
8-?
:;ü[m6:;
!
A<=/0¨©r<=¤
#
<=
—[U?D\Gªj‰ãÌÄډŠ2s@
#$!
%&#(!%)!67)
¼k8É6‹

r]ꃄm6
:;AB<=èéÚ6U?[6Ýܵo¥,s
©
!
>stƒ„¹u

!
ÁÂх”
!]!
ÉÌÁÂGõP;q
!
<=vÜÙwx¤§
¨y¶·

V]
<1
ùþõPs_zÔ_ô[vÜ
ÙwKÐba>
MhL I*/B
QP
W!^;R
Ñ
O I*/
B
QP
@Rd
[
<1
žþõPs
$Og
{U
!U.LhN%
r8
É/0õP
!
| ·
$
}i
%

W */B
QP

Ö¥Ù

8-?!
ͪ<6Fê
PMg!WMg!OMg!

€a
8-?
[/0¨©êA“

]I“
PX ##

N
#
;qª8ÉõP
!
‚êKLê
PWM %/I)
QP
!
õP
®ê
$WL iP% j
6F]Ö¥¾
^!N!PX 5


7Ö¥³
O
5ij

/0¨©ƒ]˞„L…¾
AŒ]
Q[M j
†0r
!
ݛ

!]#
ßàÑçÛ
!
™Þg2Àß
$S$%/0V$)
6&$0#%#
2Àß«›%·V
$
èÝF
%#
m‡ˆ
a$;
2(%0WX_L
—éžj{‰ß
!a$2(%0W__X
üŠû‹J
1%à
!<&())4IOW
NE¹
=*&()2@;
PN

Œ
$^hX IIkWLM II!L
!
I%
A“]<=¤
$
Ãf
MNX[;WMMWML!
l
_Ng%#
<=—
$
Ãf
PPM[XX;
WMM^PX!
l
_Ng%
Œlr­Ç]ãÌÜ]1Ô
²
!
{‰lَ
#

!
6‹lÙ@
!
—lm
$
§¨y
/[LPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP

% @%`/&
l
:)D2%/*()
–$
! GR>=
ÄÚçÛ
‘l
@$C$3$
–$

!]$
56@Z·%Ô
X(
!
#
P IB
/0¨©ƒ]
!
a
Ð
OMM
!
BOg
’sm“@µ#”•ž
!
–m‡
PM
I)!
—˜¾G#
PMM
!
B
_óž
!

PMM
!
B
†™
@
!PMM
!
B
@›šƒ>
!
–m‡
OM I)!
—˜¾aÐ
P
IB
ل
!
›‚
OM 0!
qœ¾#_ó4ßûr
!
]
!!
O MMM %/I)
QP
ª4ß
L I)
G#_456@ل
ž
WMM
!
B
]2Àß_1%
LWM )I
[GI_
!

µ#žô\¨©žEêctA“
!
’–}
8
56@
$I*
/B
QP
% Y$L k#
LWM
ZW%Z
¨©þU
!
.P56@Z·

!]%
“Ìá.
!
ê)
?()7$)V
()žr[s@e
Já.“Ì
!
“ÌÄÚx_&ãÌN.÷Ñè#–
$Ú
!
eJŸ¼
P
¼
P!
s@eJ“Ì
s@r  “ÌeJ
$Lf;Of%
vÀr x
ZbU ?()b$)V
$%f
#$ J/%E$%5.==?@@===?@@ PO_ -JLMP[XX
3(D(%0(.?==?@@=?=?@@
%&#( J/%E$%5.=?==??==?=@=?@== P[^ -JLPWLNM
3(D(%0(.?======??==?=?==
67) J/%E$%5.@?=@??==@@@ POM =7MMN[^N
3(D(%0(.@?@=@@?@==?=
%) J/%E$%5.=?=?=?@?@?==?=@==? WO_ =7M^MM[W
3(D(%0(.=@=?@=??@=??==?
PN1 J/%E$%5.?@@?=@=?@@?@@ P[_ J9LW[XM_
3(D(%0(.?@@=@=??===@@?@@=?=
!]&
5
3>=
µ#i
GR>=
ÄÚ
!
´µõP
^!PX 5
[/0¨©Ö¥ÙÑA“Ù
!
›
:)D2%/*()
–$[
@%;
`/&%($*()2
µ#/0¨©ƒ]5
3>=!
¬›¡®6I
Iß%Ô5
3>=
[
#
WXM )I
!#
WNM )I

´µ
#
WXM )IZWNM )I
ê
PhN lWhM
[5
3>=
8É¢K%
!
¢K%¢\’
GR>=
ÄÚçۑ
$@$C$3$
–$
%
h.Æ8É

!]
7Ô·
3@;83
^(
!
#_£¢K%äÌ
P
!
B!
6Faм
P
r¿s@»†“Ì
MhL
!
B$PM
!
I/&
/B
QP
%!
¢†“Ì
MhL
!
B$PM
!
I/&/B
QP
%!5>@8P
!
B!PM kb46(%WhL
!
B!-K(.9
2
MhL
!
B!
£N
.
W
T
P_
!
B
¢\;qê
_^ j
ÈD›
L I)&_^ j

OM 0!
ÊU
^M 0![W j
¤¥
P I)!OL
¦*
&[W j
á
6¤¥
PM I)
m¡§ª
PN1 %3>=
s@r 
!
¨ž
GR>=
aзͿƒ¤[üV<|A

“›_£
þF§ªj‰Þgs@[¼k©´
!

PN1 %3>=
s@r ê~À
!
]vÀ
83
äÌxIœKR;
ªêj‰2s@è¼k

îaÚ«ß_¬­[¿
vÀs@i
PN1 %3>=
[IGH•_CE;¼½v
Às@]YZ
8-?
<Ö¥Ñ#ƒ7š[¼k·

!](
<=¤
#
<=—Z·[%Ô
^(
!
#<=/0
¨©ž
P IB!^ j PW MMM k:
4ß®_ó
!
aÐ6‹
lَ
P IB!
Pi
!
 Ùµ#
PWM I)!
#K¾Pi
>‚¯èi

›
Mh^L
!
I
°±¡°&1%ž

“›
.8B
”%ԃ]r<=¤
#
<=—Z·

&
Êj
$=%

;
m
;
Ù@
$WOm[[mPhM%&$7%

;
m
;
Ù@
$NMmWMmPhM%&
²³´.eê
M lPL I)!
PMMg$=%&PL lWL I)!PMMg lN[g$=%&WL l^ M
I)!N[g lLWg$=%&^M lXM I)!LWg lM$=%
1
%µx
WNM )I&
6‹7š
XM I)&
Ψ
OM j
#
0•i6‹
#]!
56@Z·ÊìD!
€a
8-?
[A“Ù<=
/0¨©~3456@Z·9õP7š¤x,u¡D

YZ
8-?
<Ö¥
^ 5
¾
!
¿Ù<=/0¨©~
3456@Z·Œ—A“
!
<
8-?
:;<¶u
!
3
456@Z·¶—
OMg8-?
Ö¥
N!PX 5
¾
!
<=/
0¨©~3456@Z·`Œ—A“
!
Ÿd
P
#]#
<=¤
#
<=—U?ÊìD!
9iÖ¥7š
[¤x
!
A“Ù<=/0¨©~<=¤
#
<=—[Z
·Œu¡ªa
PMg! WMg! OMg8-?
:;
PX 5
¾
!
<=/0¨©~<=¤Z·Œu¡·A“Ù
&
f
PMg8-?
Ö¥Ùr<=—Z·u¡—A“Ù
!
WMg! OMg
<[
8-?
Ö¥
PX 5
¾¨©r[<=
—Z··A“Ù
!
Ÿd
P
#]$
<=—
#
<=¤ãÌÄÚ2s@¼kD!

PN1 %3>=
ê~À
!
m¡7Ô·
3@;83
1%
#$!
%)!%&#(!67)
s@]<=/0¨©
^!PX 5
GY
/NLPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP
!!
d
P!
<=/0¨©r56@
#
<=¤
#
<=—Z·
#
<=¤i<=—•[ÊìDZ<
8-?
:;[¼k©´
!
Ÿd
W
iA“j•
!
/
0¨©õP
^ 5
7
!#$
s@¼k·]
PMg8-?
<
:;ªu¡µ—
&
õP
PX 5
[/0¨©]
WMg8-?
<
:;ª¼k·u¡µ—
%&#(
s@]
WMg8-?
<ªõP
PX 5
¼k·¸—
8-?
:;;qª
!
<=
/0¨©r
67)!%)
s@¼ks@¹èu¡D
d
W!
<=¤ãÌÄÚj‰s@¼k6‹
/_LPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP
#]%
<=¤i<=—Z·•
!
<=¤i<=—[
•_9i
8-?
:;<[YZºRYZ©´
!
Ÿd
P
õP
^!N 5
[/0¨©A“ÙÑÖ¥Ù[¤i
¤8•¹èu¡CE

õP
PX 5 WMg8-?
[/0
žr¤—•—A“Ù
&PMg!OMg8-?
<[/0
¨©r¤i¤8•uÖ¥Ù

$
†,
$]!
<=èéÚ6U?õP;qƒ„
!
¤70•
¼.
!PMg8-?
:;
^ 5
¾
!
<=/0¨©r<=—
Z·u¡_3
!
><9i:;7š[¤x»¼3—


!8-?
*Eê=8<=/0¨©r<=—U
?[¶·Û

$]#8-?
ü[m6:;A<=—ãÌÄÚ[AB
!8=B
M<>ãÌÄÚ´µr[‰Š2
[(
!
]/0
¨©r
!PMg8-?
:;
^ 5
¾
!8=B
¼k·u¡_
3
!
¼.
PMg8-?
:;kÄ><=—[ãÌÄÚ
!
±f=8><=—]¨©r[U?

$]$8-?
ü[m6:;A<=/0¨©¡c\g
ó@Fã[AB
!
<=—Eêbc\Û
!
*gi
>¾×;qª<=þ~¡c\g[ó@

]
8-?
ü[m6:;;qª
!
¨©gri&[š¿ÛÑÀ
´mÝ56@[Z·äu·U?
!
ªU?·i¾×
m¹GÉÌA¾×[b›è‰
N(
!
]ÉÌÁ\¾×
¡.rgZ·¨©š¿Âµ—ÉÌÙw[»m
N

]¤§¨r
!
<=/0¨©~3456@[U
?·i
8-?
<º»•

<=¤M<=—[Ì|}
_(
!
ÎÉÌab¾
×:;7
!
<=¤mQꪤ8fbc\Þg

]
PMg8-?
:;
PX 5
¾
!
<=¤Z·u¡ªZ
!
Z

Z
¤8u¡·A“

½ 0•u½K9i:
;7š[¤x
!
¨©~[<=¤u·mQê¤8
ibbc\E›r;
!
Y¬<=¤[U?

WMg8-?
:;
^ 5
7
!
<>¨Ú6Z·Ñs@
¼k·¹èu¡›CE
&PX 5
¾
!
/0¨©r<=
—Z·u¡ªZ
!
ì
#$
s@¼k·u¡_3
!
Z

Z
¤8u¡_3
!
Äܤ†¤8K\[‰Š2
%&#(
s@¼k·`u¡_3

½RZ¼.]
WMg8-?
:;ª
PX 5
7
!
<=—ãÌÄÚÅÄY
!
ì˜Yg=8<=—]¨©r[U?
c$I$0$V!
.
‡
PM(
1#
=8+
*Vc\â<>ÆÈêÆÈâ
<>
!
@½Ç%x
WMg8-?
kÄ>
=8+
‡bc
\2[Y›
!
±f?Ü><=—ió@Y›c
!
·
A<=—†<=¤K
$
•ƒ–—
%
P (!
È­É
!
ÊËÌ
!
ÈÍÎ
h
r­ÇÁ]
<(h
øù
.
r­ÏÇ
ö÷KÂÐ
!P__X.O_Nh
W (!R/V(>h:)D/&D()I()2/604U(%/K5($)/) *()(%$2/) ) 2#(#A;
U(%0()2D(%(0U/)0(/6U2$2/24b(%2004(2/)6(G2/) E2# )G/I;
U$2b&(%$G(/6U#A2/U#2#/%$)6(02$)0$)5 2#(#AU#$&G(&E$&
G/IU/)()209(h8#A0/&8&$)28$2#/&!P_NO! WO.O^Lh
O (!
òÑ
!
ÒÓ
!
ÔYÎ
!
‡
h
ÕÖCŠ>Ä2s@
4267)P
[:
×GªÙw¯E›¼k
9(F
ØÞ]1
!WMPM!O[$N%.PW_Lh
^ (!
H’j
!
ÙÚ
!
ÛÜæ
!
‡
h
I“A<=<>¨Y›Ú6U?
Gªj‰s@¼k[AB
9(h
r­rÇ£¤
!WMPM!OL$L%.
^_h
L (!
n[[
!
H’j
!
<ÝÞ
!
‡
h
m6:;A<=~¬Ä—iè
éÚ6j‰›[AB
9(h
r­§¨…Û]£¤
!WMPM!PX
$[%.__h
X (!
ßàá
!
.âË
!
ãäå
h
ÉÌ56@GªÄÚ2Fƒ„8
æ
9(h
{çö]
!WMMN!WL$W%._Mh
[ (!
èé­
!
S¤ê
!
7ëG
h
m“@¶·ì{A
@[b݄
8=B
Y›D\ƒ„
9(h
ÉÌÐ¥]1
!WMMO!OO$W%.P_Mh
N (!
òí
!
îïð
!
ñò
h
ǛÉÌAÇ::;[B\G§¨ó
ôi…”
9(h
r­rÇ£¤
!WMPM!OL$PL%.P_P_h
_ (!<$0$/.%/2$)! 3A/C4%/5$! .5(A4V14 4`V! (2$&F&/))*
$)5 (KU%(00/) /6,R8;*&4G/0(. 6&$D/)/5 [;5;*&4G/0A&2%$)06(%$0(
6%/I#$%A%//2G4&24%(0/6)*+!,-./0. 1.0*.-,34049(F8&$)2$!
WMMM! WPM$X%.PMMXF
PM(!c$I$0$V.! 1$V#$I$c! :V(#$%$>hJ&$D/)/5;U(%/K5$0(%(;
$G2/) $0$5(2/K6G$2/) I(G#$)0I/6U&$)2G(&0$*$)02.
W
T
W
9(h8&$)28#A0/&! P__[! PPL. P^MLh
/MXPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP
,MAUDKBMP,VKDGAKKBCM1/SBCB0HK/UUI.I1/D0BC/CHGA1/DAHLACAAQNGAKK0BC
0CKIKNACK0BCBM!#$%&() *)&%+,),
c=>?d#$/G#4)! c,=>c4$)! .-><)
!
! 1.,=:B)*6(! +:=Te$)! B:>1#46$)*
$;34!0!+!,<=6>03,4,8.!,/0. 8,?0*.! 6>03. #*.?,@A<=6>03,4,8,?0*03.-)*0,3*,4! &,0B03: PMM[MM! 6>03.%
6OKDG/UD( 8O[AUD0SA.@/0245A2#((6(G20/68-?02%(00/) b$G$&)! b$G$&() $GG4I4&$2/) )54G(5 bA$) )G%($0(5 G/)G();
2%$2/) /68-?0/&42/) $)5 2#(%(&$2(5 *()(0f(KU%(00/) ) 040U()0/) /6)*+!,-./0. 1.0*.-,3404h>ADEBH.@#(G/)2()2/6b$G$&)! b$;
G$&() ) 040U()0/) /6)h1.0*.-,3404E$05(2(%I)(5 bA.8Bh@#(%(&$2(5 *()(0f(KU%(00/) E$0$)$&A`(5 bA0(I;]4$)22$2D(83h
:AKI1D. @#(G/)2()2/6U%/&)() 040U()0/) /6)h1.0*.-,3404E$0U%/I/2(5 bA8-?2%($2I()2h@() U(%G()28-?2%($2I()2U%/I/2(5
2#((KU%(00/) /68=B$)5 2#(G/)2()2/6b$G$&() ) (KU(%I()2$&I$2(%$&D$$5%/4*#202%(00hWMg8-?0/&42/) 2%($2I()2U%/I/2(5
2#((KU%(00/) /6,7?=@h=22#(0$I(2I(! 2#()G%($0(5 $G2D2A/6=8+)#b2(5 2#(U%/*%(00/6(&I)$2)*%($G2D(/KA*() bAb$;
G$&()! E#G# )54G(5 2#(2%$)06/%I$2/) 6%/Ib$G$&() 2/b$G$&)h2BCU1IK0BC. =G2D()*%(5()2) 040U()0/) /6)h1.0*.-,3404E$0
U%/I/2(5 0*)6G$)2&AD$$02%(00/6&*#2G/)G()2%$2/) /68-?0/&42/)h
A^F WBGHK(!)*+!,-./0. 1.0*.-,3404& 040U()0/) G(&0& 8-?& 0(I;]4$)22$2D(83& *()((KU%(00/)& b$G$&)& b$G$&()
HB0.!+_%#)RU[U..#+!!!+!
$
˜™$Q
!
š›œ
%

!!
ž
x—7õäöWz[
,
RôrÇY·ÄÜG÷Ñ
-
Ï]ö]KÂÐKÂÉ
!
÷ƱrÇY·ÄÜ[i
&›Ñ‘&›K
!
ø£>rÇY·ÄÜ[æñõ
#
æùÂ
&
ú¨}û>rÇ[;¬ÕF
#
›ëÕF
#
u
XÕF
#
¥\ÕFъ÷Ñ]rÇÕFr[\›
&
—éžj{‰
#
 —Mžj{‰
#
—逨û.ü
#

Q
Y‰·›÷ч{‰÷Ñ]rÇY·ÄÜr[\›
&
ÆwxrÇ!ýd‰Y·ÄÜ÷ÑGãÌéþ
#
ôhÙ
]
#
6ãÌ]‡RôY·ÄÜ÷Ñ

½®
!
Æ«þœÿ}û>ZYZ¨âY›Ú6rÇGYZÛârÇ
!
rÇÁ
#
rÇ!rÑrÚÇ
!
GrÇAæ][Y·ÄÜ

#*orÇÑÇ]j‰¯°[öƒ
#
ä]±
$͛
!
`*EêrÇÑÇ]j‰¯°[ƒ„ã
#
¤ö㐑

Îθ
#
%¶¸
#
ŠÎÆ&GÏ]«çÆ&è(

Ôþ
PWM
8

¹U̘f€›
!
·F±
.
®)ð
&
.
/
.MPM QX^MO^XMP X^MPLPXL&
Q%
.
øùSú<*êøþ
PX
f ö]KÂÐ
$PMM[P[%&
·F±
.
®)ð
&
#]
ÿ+ž?.Æ[ÆM
#
,(
#
·F./
#
-‡

/PXPW/
{
OX
ï{
PX
•
WMPP
÷
N
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! :004(!PX
=4*402! WMPP