免费文献传递   相关文献

Study on apoptosis of SMMC-7721 hepatocarcinoma cells induced by Oxytropis falcata and its preliminary mechanism

镰形棘豆诱导人肝癌细胞SMMC-7721凋亡及初步的机制研究



全 文 :½|¾¿LM±ÀÁab
1==<[[WP
ÂÃGÄÅ[Æ܃„

#
&·“
!
#
Eƒƒ
#
.“µ
#
ÂÃÃ
#
WVê
!
!
}ï8HKA¯ œ^ù8KNYåûìíî ;¹8HKYþZ8KNY78åûìíî
;¹5(<8KNYG©ÊB78ÊëjLM8!
#
œ^ }ï
WPMMW_
$
‚;
%!

&
JK½|¾¿5(RA±ÀÁab
1==<[[WP
ºÏ[TUGÄÅ[ÆÜB†

•–
.
YZÛ·[½|
¾¿5(RÖ¥
1==<[[WP
ab
W^ #
¾
!=AA
”JK5(RAabºÏ[¤ÜE›
&
\›I]ÑÕIuXvJK5(RA
1==<[[WP
ab|ì[TU
&
\›1}abßW%
1==<[[WP
abò•[D&>))(K) Sð·”W%5(RA
1==<[[WP
abÂÃ[TU

—˜
.=AA
§¨0•¼.
!
½|¾¿5(R¤Ü
1==<[[WP
ab[ºÏ
!
>SRK|Ô[<
¦@›
./(G#02OOWLN
·{0•u½
!
˽|¾¿5(RÖ¥[abKR™ ÂïeÑ X01[D
½|¾¿5(
RE›
1==<[[WP
ab¾*JK9
@
P
•ab.uº™
!
ÂÃab[Hµ3#
!
>‡S<¦@›

—™
.
½|¾¿5(R¤
Ü
1==<[[WP
ab[ºÏ
!
iªLMabò•RBÑÍab|ìãD\f“³abÂÃ[כè‰

_&î|ŷЃ
„щ£½|¾¿m‚‡[E›ÆÜ

$
ƒ„…
%!
½|¾¿
&
5(R
&1==<[[WP
ÀÁab
&
abÂÃ
$
8†$f
%!WMPMM[WMMM_
$
‡ˆEq
%!
­æléö]stuv
$OM_MWMPW%&
À]Rr…Ç2
uv
$C7M_MWX%
$
‰€>Š
%!
!
XI.
!A(&. $MWL%NX[_NWNP!-!
Àâ}
!-!!
ÀÁNéÃ[µ›‚‡g|
!
>‡]#ÐÃ
sÁªrM{ƒ

ÀÁ[usÑÐÃsB]÷ºa
P(

Lçêo
!
6Pè0![…”ãä8¨
K^›µ\›tu

”÷;è‰ÀÁ6NÆÜ[ƒ
„¼.
!
abºÏÑÂÃÇAN‚‡|Ú[i&È
Y
W(

LM‚‡abÂÃèMÚêã䂇[Š
õ-
#
Šå¼

½|¾¿
QK-!:)*987.67!7 74)*(
N
|Üi&[&Ç
!

)
XÇgþ
*
[Pf
!
‚&Z
èãÌ{
#
(R
#
 ¡‡Ú6
!
wèó}l¼
#
Þem
©
#
oQ
#
ãnOA‡Þé
O(

¤KDك„
^¼
.½|¾¿wèþ®m‚‡×›
!
ªžÑ5(
RØIwè¤Ü±ÀÁab
1==<[[WP
ºÏ[×
›

¤7D›
=AA
”JKYZÛ·[½|¾¿5
(RA
1==<[[WP
ºÏ[TU
&
\›I]ÑÕIu
XvJK5(RA
1==<[[WP
ab|ì[TU
&
›1}abßW%
1==<[[WP
abò•[D&
>))(K)ð·”W%5(RA
1==<[[WP
abÂÃ[TU
!
ê½|¾¿[î|ф—µ
o¦)

!
ÁÂiçÛ
! !^
ÁÂ
!
½|¾¿
WMMN
÷
PM
²øl)&Ï
Zlã(ò™T
!
-ýùr…Ç|]Ç]è$õù
Ž/ÕÔ

! #^
çÛ
!
xüŒs
PQs
%ü’s–›Q
$=AA! 1*I$%&
üŒs.]
$>I%(0<
G/%&R=-=
õ=s
$@dG/!
Ù­
%&
TÏQó
$J71!
…ç–µçãÌNô–$
%&
WÝt®
$A%BU0)!
@dG/%&
çV—
#
ÂV—
$
óúÒm…Çãtè#–
$
%&
b@wc
$?$.T
O
!
_&çۖå
%&871 $
²
øz{{ãÌNôè#–$
%&>))(K) SÂÃ
çÛ¨
$
ýùósãÌè#–$
%&8;$1*I$%& ./<
(G#02OOWLN
·{çÛ¨
$
R)8ãÌ÷у„²
%
! $^
ßà
!T
W
abõ=0
$JT3=>! A.-3=T%&
7/<3$5 XNM
®·¹ÛW%ß
$
Ù­
%& 7+LPAJ
ÕI
uXv
$T&BIU40%&;+LP
I]uXv
$T&BIU40%&
1
}abß
$J>1$)2/
(
! 7R%
! %^
abaGabõ=
!
±ÀÁaba
1==<
[[WP
-ýùr…Ç|]s©…]èþ.1Ž/1
Í
1==<[[WP
ab›Z
PMm
U×TÏQó
#
çV
—
PMM ,/IC
QP
!
ÂV—
PMM
!
*/C
QP
[
R=-=
õ
=s
!
]
O[ j!Lm T
W
ïqªõ=

/WXOP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
#
…”
# !^
½|¾¿5(R
$AJTJ%
[ÜÝ
!
#½|¾¿Õ
ÇÁ
PMM *!
Ë
_Lm!LMm
“Ž¦5µ#
!
»MÈH“Ž
!
a
Wm ?$T.
ñsñl
!

!
TsaÐ
Pm .&
ñs

U.L!
6
#
“@“•¦5Ë#
!
Ä>Ë#s
!
<
¢±Õ
!
&½|¾¿5(R
!
&sê
Wh[Lm
V²&5
(RØIîÉ=%Ô
!
ª=ê
NOh[m
# #^
GF[áÜ
!
P#½|¾¿5(R2·
!
,

R=-=
õ=sêsY
!
üŒs.]
$R=1T%
º
ñ
!R=1T
Y<
MhLm
¡Tä3
!^ j
AŒÝ›

# $^
abºÏ¤Ü§¨
$=AA%!
#A(ãx•a

MhWLm
WÝt®n\¾
!
›õ=s;­égÚ
Habbs
!

W iPM
L

ZIC
[3† 
_X
 õ

!

_M
!
C!
õ=
W^ #
ÔabSõ¾
!
§¨Ù
6ŒaÐ
PM
!
C
YZ<[oçs
!
ÍaG¾¿o
çs[Y<6Œê
WL! LM! PMM! WLM! LMM I*/
C
QP
!
ctA“ÙaЇþU[,Qóõ=s
!
¡›
A“ÙaÐY<ê
^ I*/C
QP
[’¹ñs
!


X
 

abaÇõ=
W^ #
¾
!
7 aÐ
WM
!
C
Š¤áÜ[Z
L */C
QP
=AA
ñs
!O[ j
8Æõ=
^
#!
b_ós
!

PMM
!
CR=1T!
–«
!
á6ñl0
Û
!
›®·¹ÛW%ß%Ô
^_M )I
Ö¿ [GI
_
5!
.PabŒ×s

abŒ×s
Y5
§¨Ù
Z5
A“Ù
iPMMm
# %^
jCJ£uXvJK
AJTJ
Aab|ì[T
U
!
Vãx±|Ô3[ab›WÝt®n!
é
gÚHbíab
!

P iPM
^

ZIC
[3† 
W^
 õ=«
!
6ŒáctA“ÙÑÛÇÙ
!
Ôab
Sõ¾
!
aoçsÖ¥
!
ÍoçsY<ê
MhML!
MhM[L! MhP */C
QP
!
A“ÙÛÜZ‡þU[
871
aÇE›
W^ #
¾
!
]jCJ£uXvªJKõ= 
~abSõãxG|ì[D\®´

# &^./(G#02OOWLN
ÕI·{JKab*|ìD
X(
!
#A(ãx•ab7 
L iPM
L
ab[3
† 
X
 õ=«
!
 r³Ø^ÐË#@U3Ö
¥[6¯÷r
!
Ô
W^ #
abTr¾
!
aoçsÖ¥
!
ÍoçsY<ê
MhML! MhM[L! MhP */C
QP
!

ٛZèjZ<[
R=1T
Ö¥

aÇÖ¥
W^ #
¾
!
G¶õ=s
!

MhL IC
ùÔs
!
ùÔ
PM I)
ªùÔs
!
›
871
W
©
!
75
O I)!
G¶sþ

U7›ˆßÜÊ


MhL IC./(G#02OOWLN
·{
s
!
·{
L I)
›ˆßÜÊ
W gO I)
ª·{s
!
›
871
J
Mh_m ?$&
W
©
!
75
O I)!
G¶s
þ

à
P
àmÕIVUürs`÷r_
!
¯_S
èab[¯÷r
!
Ãab†Aürs
!
¶·¸¹§
Ñ

£ÕIuXvªJK
!
£Áx
OLM )I!

Áx
^XM )I
JKab~[ab*|ëGÕI´
[D
²è§¨ij
O
5

# ^
1}abÑW%
AJTJ
Aabò•[TU
!
#A(ãx•ab7 
P iPM
X
ab[3† 

X
 õ=«

6ŒáctA“ÙÑÛÇÙ
!
Ío
çsY<ê
MhML! MhM[L! MhP */C
QP
!
A“ÙÛ
ÜZ‡þU[
871
aÇE›
W^ #
¾
!
Uabõ=
sGK±Ä24ߨ~
!871
\USõab
P
5
!
›
WÝt®n\ab

abn\¾
!
aÐH®[ab
õ=s
!
C‘
!
K^94ߨ~
!P MMM %/I)
QP
4
ß
L I)!
b_ó
!
H®ab

֛
871
\U
W
5
!
›
QWM j
³€[
[Mm
“ŽùÔ
PW #
_
!
ÔW

_
Æh֛
871
\U
W
5
!
é¾aÐ
P */C
QP
[W/ì
$8;%
·s
WL
!
C!
£
^ j
¸I·{
OM I)
1
}abß6‹abò•
!
75.(ab
P iPM
^

!

=TRJ;A
Ñ
-CC ,`-1A
6‹¸q6‹

# (^>))(K) Sð·çÛ¨W%abÂÃ
!
#A(ãx•ab7 
P iPM
X
ab[3† 

X
 õ=«

6ŒáctA“ÙÑÛÇÙ
!
Íoç
sY<ê
MhML! MhM[L! MhP */C
QP
!
A“ÙÛÜZ
‡þU[
871
aÇE›
W^ #
¾
!
Uabõ=sGK
±Ä24ߨ~
!871
\USõab
P
5
!
›WÝt
®n\ab

abn\¾
!
aÐH®[abõ=s
!
C‘
!
K^94ߨ~
!P MMM %/I)
QP

L I)!
b_ó
!
H®ab
!
›
871
««ibab>.(

#
L iPM
^
gPM iPM
^
ib[ab
!P MMM %/I)
QP

L
I)!
b_ó
!

LMM
!
C>))(K) S0Äs«
«ibab

Ö6ŒaÐ
L
!
C>))(K) Si
8;!
««C‘

`@
$WM gWL j%
¸I\b
PL I)
1}abßW%ÂÃabÐ6s

›
>))(K) S<
J;A
Ñ
8;
·{¾
!
»[×abY<
>))(K) S<
J;A
Ñ
8;
·{
$ O`%&
ÂÃP•[abè<
>))(K)
S·{
!8;
·{Sœ›
$`^ %&
ƒÐabÑÂÃ
­•[ab*Z7<
>))(K) SÑ
8;
·{
$ W`% P`
ê‹*2Â~[W%-C

# )^
ã.6‹
!
²è[()Œ›
1811 PPhL
ã.
¸q6‹
!
›HY—…C6‹”6‹ÙšCE[u
¡›

0•Œ›
Kp9
¼½
!?qMhML
êCEu¡

/OXOP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
$

$ !^=AA
”JK
AJTJ
A
1==<[[WP
ºÏ[TU
!WL! LM! PMM! WLM! LMM I*/C
QP
[½|¾¿5(
RE›
1==<[[WP
ab
W^ #
¾
!
Œg¤Üabº
Ï
!
>‡S|Ô[<¦@›

¡›ÇabŒ×s
ê
^XhW^m!
ÛÇÙabŒ×s6Œê
_LhWWm!
_WhLXm! [MhLWm! OXhXLm! WLhXOm
ªr
!PMM!
WLM! LMM I*/C
QP
[ÇÌAab[ºÏ¤ÜE›i
A“ÙjHèu¡›CE

ÇÌ[
;
LM

MhPNL
*/C
QP

Ç̤ÜabºÏ[E›*gNm¡¤Ü
abãxJLMabÂÃ;§R[

ê#
!
*î|
ф½|¾¿5(RNgLMabÂÃ

$ #^
jCJ£uXvJK
AJTJ
Aab|ì[T
U
!
jCJ£uXvªJKÛÇÙiA“Ùab
[ãx|ì

RajZHµ
R=1T
õ=s[A
“Ùabbþ|
!
ãx>?3®
!
ô”%f«>JÏ
xf
!
I†!
&
faÐYZ<[½|¾¿5(R
[ab¬ÇÌ!
ab
(·.ueA“Ù
!
I†ªð
!
abǪSõã
x[¯›
!
‡ÂÃãs3#
$
ab;ƒ
!
|ì-X
|\GDi
!
b‚`¢
!
bþ¢6
!
·{Y°ïëq
®
!
>èÂÃ6þ|ڇ
%
¬CÇÌ<[3#
!
#Råûa.u
!
wè|Ô[·é‰F
$
d
P%
>h
A“Ù
&7hMhML */C
QP
Ù
&
hMhM[L */C
QP
Ù
&RhMhP */C
QP
Ù
$
d
W
Z
%
d
P!
jCJ£uXvª
AJTJ

1==<[[WP
ab
W^ #
¾[ab|ì“r
$ $^./(G#02OOWLN
ÕI·{JKab*|ìD!
Ë
./G#(02OOWLN
ÕI·{¾
!
ÕIuXvªÃA
“Ù
1==<[[WP
abXž
!
*|ìaf
!
*YC

!
3Œ‘|í
&
fÛÇÙY*Ã.u[ÂÃa
b
.
abX`¢
!
ab*jAD6
!
ÕI.uºQ
&
a
b*·{Yã<®
!
¼Rê*ù¢Rå
$
d
W%&
d
WR
u½#´ÕI
!
*Ãab~ÏË|ÚÂÃ6
þ
!
]#Û·ÇÌE›ªabôÚ,ž*01

d
W!AJTJ

1==<[[WP
ab
W^ #
¾
./(G#02OOWLN
·{ÕIuXv“r
$ i^ MM%
$ %^AJTJ
Aabò•6*[TU
!
iA“ÙjH
!
YZÛ·
$MhML!MhM[L!MhP */C
QP
%
[
AJTJ

1==<[[WP
ab
W^ #
¾
@
M
Z@
P
•ab[HµŒè²
ºa
!
-
OXm
ºa9
ONhWLm!X^hOWm![^hXXm!
#<
7[Hµ.u#A“Ù[Hµ
!
SRKiÇÌ<
[¦@23
&1
•abHµY.uðê
!@
W
Z=
•a
bHµäê<®Œ|Áðê
$
¼
P%
¼.½|¾
¿5(RVabò•RB]
@
P
•

¼
P!
YZÛ·
AJTJ
A
1==<[[WP
abò•6*[TU
AJTJ
Z*/C
QP
abò•6*
Zm
@
M
Z@
P
@
W
Z=
1
M OXhMM WWhOO ^PhX[
MhML ONhWL ^^hWL P[hLM
MhM[L X^hOW PMhP[ WLhLP
MhP [^hXX Xh_^ PNh^M
$ &^AJTJ
AabÂÃ[TU
!
D›1}abßÔ
·W%ÂÃab[(·;SR½|¾¿5(R[L
MabÂÃE›

0•¼.
!MhML */C
QP
[5(R

1==<[[WP
ab¾è
WWm
[abãÂÃ

ÎÛÇÛ·ºa9
MhM[L */C
QP
7
!
 ¡
XNm
[a
bãÂÃ

f
MhP */C
QP
[5(RE›
1==<
[[WP
ab¾Yè
[Mm
[abãÂÃ

%
†,
R􃄼.
!
½|¾¿5(R[m‚‡E›
/^XOP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP
*gN-ªr[ãÌכÌY²}M[
!
ûEWX
—
#
·Â$—ÑtH—‡
[(

¤ƒ„¼.
!
½|¾
¿5(R*èé¤Ü
1==<[[WP
ab[ºÏ
!
>
SRK|Ô[<¦@›
&./(G#02OOWLN
§¨0•

!
˽|¾¿5(RÖ¥[abKR™ Â
ïeѠX01[D
ꐃ„ªLMabÂ
Ã[ÆÜ
!
¤ƒ„m¡abò•§¨Ñ
>))(K) S<
J;AZ8;
𷧨«îɐÄÅ[ÆÜB†

abãxRB]
@
P
•NLMâ
ULO
¦@›
R?>
hif“³[
ULO
A
UWP! =5IW
Ñ
7$K
[·¦!
“³
UWP
Ñ
D;
[¦\_A
!
龓³
RD
כªAÑabò•RB
N(

’;’™[ƒ
„u½LM‚‡abãxò•[RBNŠâm‚‡
Çã䂇[Šõ-

½|¾¿5(RE›
1==<[[WP
ab¾*JK9
@
P
•ab.uº™
!
Â
Ãab[Hµ3#
!
>‡S<¦@›

-#ç%
½|¾¿5(R¤Ü
1==<[[WP
abºÏiªL
Mabò•RBÑÍab|ìãD\f“³ab
ÂÃ[כè‰

Y#
!
_&î|ŷЃ„щ
£½|¾¿5(Rm‚‡[E›ÆÜ

$
‹ŒŽ
%
P (!8$%V) R=!7%$BJ!J(%&$B9!(2$&h-02I$2)*2#(F/%&5 G$)G(%
d4%5(). @&/d/G$) WMMM 9(h;)29$)G(%! WMMP! _^$W%. PLOh
W ( !7%(4#$#) D! C/)*(%G# A! 1G#%I$G#(%8hRB0%(*4&$2/) /6
*%/F2# 6$G2/%0*)$&)*) #4I$) #(U$2/G(&4&$%G$%G)/I$9(h
T)G/*()(! WMMX! WL$W[%. O[N[h
O (!
­ær…Ǩ¥2rΤXWwä
:
rΤX
:
&Çï
=(:
_&
.
_&ö]÷ÑKÂn
!WMMW. XLNh
^ (!
È××
!
XI.
!
þY
!
‡
:
½|¾¿µ#Ìm‚‡×›[þ
®§¨ƒ„
9(:
Ç]iÞ߃„
!WMPM!PN$W%. POLh
L (!
XI.
!
È××
!
þY
!
‡
:
½|¾¿A
1==<[[WP
ÀÁab
ºÏG
==8¼k[TU
9(:
r­rÇ£¤
!WMPM!OL
$P_%.PWW[h
X (!3$I/)(5$7=! 8y%(bG2E$2/) /6U%/2() V)$0(
6/%U%/2(G2/) /6G(&0$*$)02$U/U2/00)54G(5 dB2#(II4)/<
04UU%(00/%U%/5*/0) 9(:7/G#(I8#$%I$G/&! WMMW! XO$O%.
^XO:
[ (!C((a=! CIRa! #/.9! (2$&h;)54G2/) /6G(&GBG&($%<
%(02) U%/02$2(G$)G(%G(&0dB2#(5(2$%BG/IU/4)5 0/&]4%2<
*()) 9(h9=(5 J//5! WMM_! PW$P%. Nh
N (!S(%I(4&() D!S$) 7/GV02$(&(R3!7(%)(I$) e?hA#(G(&GB<
G&(. $%(E(F/6%(*4&$2/)! 5(%(*4&$2/) $)5 2#(%$U(42G2$%*(20
) G$)G(%9(h(&8%/&6! WMMO! OX$O%. POPh
=DIHF BC/MBMDBK0KBL=>>2-[[WP EAM/DBT/GT0CB./ TA1K0CHITAH
NF A@(1.$%/20)4010 /CH0DKMGA10.0C/GF .ATE/C0K.
.-?+)*! a>?@@4$)*I)*
!
! e.>?@J$)*6$)*! c>?@=)*B$)! .-a4$)B4$)! >;7$/G#$)*
!
$;7(D(3 E(F1:9!-)82,(191#1+6(1! L7(39/ T1-=7>):7!):-)82,(191#1+6(1?:)619(3! T1-=7>):7!):-
)8&!7!15+0(9!:7!)( )8@:7+!)(7.2,(191#1+6(18):&!7(+7:+J7!)( )82,(191#1+6(1?:)619(3! <(3(11:(3 21(!1:)8
&!7!1#(9!:-)8<+/67!)( 8):&!7(+7:+J7!)( )82,(191#1+6(1?:)619(3! ;7(D(3 WPMMW_! 2,(7%
6NKDG/TD(8N]ATD0RA. A/(K$I)($U/U2/00/61==<[[WP #(U$2/G$%G)/I$G(&0)54G(5 dB2/2$&6&$E/)/50/6QK-!:)*987.B
67!7 $AJTJ% $)5 20U%(&I)$%BI(G#$)0Ih>ADEBH. 1==<[[WP G(&0F(%(2%($2(5 6/%W^ # F2# AJTJ) 56(%()2G/)G()2%$2/)0h
;)#d2/) /) U%/&6(%$2/) /61==<[[WP G(&0F$0$00(00(5 dB=AA$00$BhA#(I/%U#/&/*B/62%($2(5 1==<[[WP G(&0F$0/d0(%E(5
dB/U2G$&IG%/0G/U(h-6(G2/6AJTJ/) 2#()4G&($%I/%U#/&/*B/6G(&0F$0$)$&Bb(5 40)*./(G#02OOWLN 02$))*dB6&4/%(0G()G(
IG%/0G/U(h>))(K) S/6$U/U2/00) 1==<[[WP G(&0$)5 G(&GBG&($)$&B00h:AKI1D.A#(%(04&20/6=AA$00$B0#/F(5 2#$2AJTJG/4&5 )54G(GB2/2/KG2B
) 1==<[[WP G(&0) $5/0(<5(U()5()2I$))(%h./(G#02OOWLN 02$))*$)$&B00)5G$2(5 2#$2AJTJG$40(5 2BUG$&G#$%$G2(%02G0/6
$U/U2/2GU%/*%$II(5 G(&5($2#! 04G# $0G(&0#%)V$*(! $U/U2/2Gd/5B6/%I$2/) (2GhJ&/FGB2/I(2%G$)$&B005(I/)02%$2(5 2#$2
AJTJG$40(5 $5/0(<5(U()5()2$U/U2/00/61==<[[WP G(&0$)5 $%(02(5 G(&GBG&() @
P
U#$0(h2BCT1IK0BC.;204**(02(5 2#$2AJTJ
)#d2U%/&6(%$2/) /61==<[[WP G(&0dB)54G)*$U/U2/00/62#(G(&0$)5 $%(02)*G(&GBG&() @
P
U#$0(h
aAF WBGHK(!QK-!:)*987.67!1& 6&$E/)/50& #(U$2/G$%G)/I$1==<[[WP G(&0& $U/U2/00
HB0.!+ %^#)QT]T..#+!!!+#&
$
$Q
!
¿ÀÀ
%
/LXOP/
{
OX
ï{
PM
•
WMPP
÷
L
²
!!!!!! ! ! ! ! ! !!!! ! ! ! !
S/&hOX! ;004(!PM
=$B! WMPP