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作 者 ：周延萌,刘玉波,高允生

期 刊 ：中国中药杂志 2010年 35卷 21期 页码：2923.

关键词：丹参酮；维甲酸；骨质疏松；小鼠；

Keywords:tanshitone, retinoic acid, osteoporosis, mice,

摘 要 ：目的: 研究丹参总酮对维甲酸致小鼠骨质疏松的防治作用,并探讨其主要作用机制。 方法: 维甲酸(90 mg·kg^-1)致小鼠骨质疏松模型,丹参酮(40,80,160 mg·kg^-1)灌胃给药,实验过程监测体重,2周后以比色法测定血清钙、磷含量和碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)活性,竞争放射免疫法测定血清中骨钙素(BGP)和雌二醇(E₂)含量,取股骨进行骨组织形态学观察。 结果: 维甲酸可引起高转换型骨质疏松,丹参酮可升高E₂值,降低P,ALP,TRAP,BGP水平。 结论: 丹参酮对维甲酸致小鼠骨质疏松有一定的防治作用,其机制可能与其减缓雌激素水平降低,抑制骨高转换有关。

Abstract:Objective : To observe the prevention and therapeutic effects of tanshitone (TAN) on retinoic acid induced osteoporosis in mice. Method : The mice osteoporosis was induced by given retinoic acid intragastrically for two weeks. The histomorphological features of bone were observed and biochemical indexes in serum (Ca, P, ALP, TRAP, E₂, BGP) were determined after the mice were given TAN at the dose of 40, 80, 160 mg·kg^-1 respectly. Result : Tanshinone can induce high conversion of osteoporosis. The levels of P, ALP, TRAP and BGP in the TAN groups were lower than the model group, while the E₂ level was higher than the model group. Conclusion : Tanshitone can prevent the loss bone in the experimental mice. The machanism may be that it improves the level of estrogenic hormone and inhibits the high bone turnover.

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