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作 者 ：许惠玉,陈志伟,周丽,牛建昭

期 刊 ：中国中药杂志 2010年 35卷 24期 页码：3377.

关键词：赤芍总苷；K562；非受体依赖途径；

Keywords:total glucosides of Radix Paeoniae Rubra, K562, non-receptor-dependent pathway,

摘 要 ：目的: 研究赤芍总苷诱导K562肿瘤细胞凋亡的信号途径及相关基因变化。 方法 :通过MTT,流式细胞仪,实时定量PCR,Western-blot等方法研究caspase-3 mRNA,caspase-9 mRNA,caspase-8 mRNA,细胞色素C,Bcl-2,Bcl-Xl,Bax蛋白水平的表达。 结果 : MTT法显示赤芍总苷可抑制K562细胞生长,具有量效-时效关系,caspase-3 mRNA, caspase-9 mRNA增加,细胞色素C含量增加,caspase-8 mRNA无显著变化。调节基因蛋白表达也有所变化, Bcl-2,Bcl-Xl下调, Bax上调。 结论 :TGC主要通过线粒体途径,当细胞受到死亡信号刺激,与Bcl-2或Bcl-Xl蛋白相结合的Bax蛋白就会被置换出来,线粒体膜通透性增加,释放出一系列物质,导致K562肿瘤细胞凋亡。

Abstract:Objective : To study the total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes. Method : By MTT and flow cytometric, real-time quantitatie polymerase chain reaction (PCR) and Western blot methods researched the level of genes and proteins. Result : The total glucosides of Radix Paeoniae Rubra could inhibit K562 cell growth by MTT method, there was the relationship of concentration-time between them, TGC prompted K562 cell translocation of phosphatidylserine, may be apoptosis through non-receptor-dependent pathways because caspase-3 mRNA, caspase-9 mRNA and cytochrome C increased, caspase-8 mRNA had no significant change. The expression of Bcl-2 protein and Bcl-Xl protein could decreased, the expression of Bax protein could increased by regulating gene expression. Conclusion : TGC induced K562 cell apoptosis that might be through the mitochondria pathway, when cell apoptosis occured, Bcl-2 or Bcl-Xl proteins combination of Bax protein would be displacement, then the mitochondrial membrane became permeable to release a series of material, then lead to cell death.

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