期 刊 ：中国中药杂志 2008年 33卷 06期 页码：686.
关键词：子宫内膜异位症；雌激素；类固醇类产生因子-1；小鸡卵清蛋白上游启动子转录因子；17-β-羟甾类脱氢酶；含药血清；
Keywords:endometriosis, estrogen, steroidgenic factor-1, chicken ovalbumin upstream-transcription factor, 17-beta-hydroxysteroid dehydrogenase, medicated serum,
摘 要 ：目的:探讨加味三棱丸(SLW)抗子宫内膜异位症内膜雌激素生成的作用机制。方法:以SLW含药血清作用于体外培养的内异症在位内膜细胞,RT-PCR检测子宫肌瘤组、给予SLW含药血清前后内异症组类固醇类产生因子-1(steroidgenic factor-1,SF-1)、小鸡卵清蛋白上游启动转录因子(chicken ovalbumin upstream-transcription factor,COUP-TF),17-β-羟甾类脱氢酶1(17-beta-hydroxysteroid dehydrogenase 1,17-β-HSD 1)和17-β-羟甾类脱氢酶2(17-beta-hydroxysteroid dehydrogenase 2,17-β-HSD 2)mRNA的表达。Western blot检测上述各组SF-1和COUP-TF蛋白的表达。结果:子宫肌瘤对照组SF-1,17-β-HSD 1 mRNA及SF-1蛋白的表达明显低于内异症内膜组,而COUP-TF,17-β-HSD 2 mRNA及COUP-TF蛋白的表达明显高于内异症在位内膜组,差异有极显著性(P<0.01)。以内异症在位内膜为对照,给予对照组SLW 5.0,2.5 g.kg-1.d-1 大鼠含药血清(体积分数为0.1)作用48 h后,COUP-TF mRNA和蛋白、17-β-HSD 2 mRNA的表达明显高于对照组,而SF-1 mRNA和蛋白、17-β-HSD 1 mRNA的表达明显低于对照组,差异有显著性或极显著性(P<0.05或P<0.01)。SLW 1.25 g.kg-1.d-1组COUP-TF mRNA和蛋白的表达水平明显高于对照组,SF-1蛋白和17-β-HSD 1 mRNA表达明显低于对照组(P<0.01),而SF-1,17-β-HSD 2 mRNA的表达却无明显改变。结论:加味三棱丸可降低子宫内膜异位症在位内膜细胞分泌雌二醇的水平,与其抑制SF-1和17-β-HSD 1,增强COUP-TF和17-β-HSD 2的表达密切相关。
Abstract:Objective: To explore the mechanism of inhibitory effect of SLW on estrogen production by endometrial cells of endometriosis.Method: After the model of eutopic primary cultured endometrial cells of endometiosis and hysteromyoma in vitro was successfully established,the changes of steroidgenic factor-1(SF-1),chicken ovalbumin upstream-transcription factor(COUP-TF),17-beta-hydroxysteroid dehydrogenase 1(17-β-HSD1)and 17-beta-hydroxysteroid dehydrogenase 2(17-β-HSD 2)mRNA were detected by RT-PCR before and after treatment of medicated serum of SLW.The changes of SF-1 and COUP-TF protein were also observed by western blot synchronously according to the same treatment method mentioned-above.Meanwhile,the data of hysteromyoma group was obtained from the above experiments.Result: The expression of SF-1 mRNA and protein,17-β-HSD1 mRNA was weak,but COUP-TF mRNA and protein,17-β-HSD2 mRNA was remarkable in Hysteromyoma endometrium,as compared with those of endometiosis,which was taken as control group(P<0.01).After the 48 hours‘ treatment of medicated serum of 5.0,2.5 g·kg-1·d-1 of SLW,the expression of COUP-TF mRNA and protein,17-β-HSD 2 mRNA was found significantly increased,but SF-1 mRNA and protein,17-β-HSD 1 mRNA was decreased in contrast to the control group(P<0.01 or P<0.05).Although the expresson of COUP-TF mRNA and protein was increased,SF-1 protein and 17-β-HSD 1 mRNA was decreased in 1.25 g·kg-1·d-1 medicated serum group,compared with those of the control group(P<0.01),the low dose group had no apparent inhibitory effect on the expression of SF-1,17-β-HSD 2 mRNA.Conclusion: The medicated serum of SLW could inhibit the secretion of estradiol in eutopic endometrial cells of endometiosis,and its mechanism might be associated with combined action of inhibiting expression of SF-1,17-β-HSD 1 and up-regulating expression of COUP-TF,17-β-HSD 2.