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作 者 ：鲍天冬,张英丰,李玉洁,杨庆,翁小刚,董宇,朱晓新

期 刊 ：中国中药杂志 2010年 35卷 9期 页码：1193.

关键词：LC-MS；炒白芍提取物；芍药苷；药代动力学；

Keywords:LC-MS, Radix Paeoniae Alba extract, paeoniflorin, pharmacokinetics,

摘 要 ：目的: 建立LC-MS测定大鼠血浆中芍药苷含量的方法,并探讨其在大鼠体内的药动学行为。 方法 :大鼠灌胃不同剂量的炒白芍提取物后不同时间点采血,LC-MS法测定血药浓度,拟合药动学模型并计算相关药动学参数。 结果 : 芍药苷在2.5~500 μg·L^-1(r=999 4)线性关系良好。方法学考察均符合要求。大鼠灌胃炒白芍提取物0.2,0.4,0.8 g·kg^-1后,半衰期基本一致, AUC与给药剂量之间呈现良好的线性关系,属于线性动力学过程。 结论 :该法专属性强,灵敏度高,可用于芍药苷的体内定量分析,芍药苷在大鼠体内动力学过程符合一级吸收二室模型。

Abstract:Objective : To develop a LC-MS method to determine paeoniflorin concentration in rats plasma. The method was applied to investigate pharmacokinetics of paeoniflorin in rats in vivo. Method : Blood samples were collected at different time after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0.4, 0.8 g·kg^-1. The paeoniflorin concentration in plasma was determined by LC-MS method. Pharmacokinetic parameters were fitted by WinNonlin 5.1 software package. Result : The linear range and the average recovery of paeoniflorin were 2.5-500 μg·L^-1 (r=999 4). and more than 80％(n=5), respectively. The inner- and inter-days precision were both less than 15%. The T_1/2 was similar. The relationship between dose and AUC showed good linearity. Conclusion : The method described in this report has high sensitivity and selectivity, and was suitable for pharmacokinetic study of paeoniflorin. The kinetic process of paeoniflorin in palsma showed two-compartment model after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0.4, 0.8 g·kg^-1 to rats.

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