Abstract:Objective: To study the aqueous constituents of Houttuynia cordata. Method: Various columns including Diaion HP-20, Sephadex LH-20, ODS and silica gel were employed for the isolation and purification of compounds from H. cordata. The structures of the compounds were identified by physiochemical properties and spectral analysis. Result: Five compounds were isolated, and their structures were identified as chlorogenic methyl ester (1), (E)-4-Hydroxy-4-[3′-(β-D-glucopyranosyloxy) butylidene]-3, 5, 5-trimethyl-2-cyclohexen-l-one (2), 2-(3, 4-dihydroxyphenyl) ethyl-β-D-glucopyranoside (3), p-hydroxyphenethyl-β-D-glucoside (4), 4-(β-D-glucopyranosyloxy)-3-hydroxy-Benzoic acid (5). Conclusion: All compounds were isolated from this plant for the first time.