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期 刊 ：中国中药杂志 2006年 31卷 04期 页码：323.

关键词：苦豆子；结肠炎；超氧化物歧化酶；一氧化氮；髓过氧化酶；

Keywords:Sophora alopecuroides, colitis, superoxide dismutase, nitrigen monoxide, myeloperoxidase,

摘 要 ：目的:观察苦豆子总碱对实验性结肠炎大鼠的结肠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、髓过氧化酶(MPO)表达的影响。方法:将SD大鼠随机分成6组,采用三硝基苯磺酸(TNB)灌肠法,制备实验性结肠炎大鼠模型。第2天开始灌胃,3周后测定结肠黏膜组织中SOD活力、MDA含量、NO含量、MPO活力。评价大鼠的症状和结肠组织学变化。结果:造模组大鼠结肠炎黏膜SOD值显著低于正常组,MDA,NO,MPO值显著高于正常组(P<0.05)。各治疗组均能升高SOD值,降低MDA,NO,MPO值(P<0.05),改善大鼠的症状和结肠组织学损伤(P<0.05)。结论:苦豆子总碱可能通过抑制氧自由基反应,抑制NO生成,抗炎作用来改善或减轻结肠炎症状。

Abstract:Objective: To investigate the effects of total base of Sophora alopecuroides on expression of SOD,MDA,NO,MPO in rats with experimental colitis(UC).Method: SD rats were randomized into 6 groups and colitis was induced by administrating 2,4,6-trinitrobenzesulphonic acid(TNB) recatlly in rats.Rats were given drug by stomach after the first day for 3 weeks,and sacrificed at 23~(rd) day to determine the content of MDA and activities of SOD,NO,MPO in colonic mucosa,and to estimate the symptom and colonic histology. Result: In UC rats,SOD was significantly decreased whereas MDA,NO,MPO were increased when compared with the normal group(P<0.05).In all treatment groups,Sophora alopecuroides could increase SOD,decrease MDA, NO,MPO(P<0.05),and improve the symptoms and histological damages.Conclusion: The total dose of S.alopecuroides may ameliorates or alleviate the symptoms of UC through inhibiting oxygen free radical reaction,decreasing the generation of NO and exerting anti-inflammatory effects.

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