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期 刊 ：中国中药杂志 2008年 33卷 12期 页码：1422.

关键词：鬼箭羽；化学成分；抗肿瘤；

Keywords:Euonymus alatus, chemical constituents, antitumor,

摘 要 ：目的：研究鬼箭羽Euonymus alatus的化学成分及抗肿瘤活性。方法：利用柱色谱分离，经理化常数测定，结合¹H-NMR,13C-NMR,EI-MS鉴定结构，利用台盼蓝法测试部分化合物的体外癌细胞生长抑制活性。结果：从鬼箭羽的95%乙醇提取物的石油醚、醋酸乙酯萃取层中共分离得到10个化合物，分别鉴定为(+)-松萝酸(1)，苯甲酸(2)，2-羟基-4-甲氧基-3,6-二甲基苯甲酸(3)，羽扇豆醇(4)，3β-羟基-30-降羽扇豆烷-20-酮(5)，(2R,3R)-3,5,7,4′-四羟基二氢黄酮(6)，山柰酚(7)，5,7,4′-三羟基二氢黄酮(8)，槲皮素(9)，胡萝卜苷(10)。活性研究表明，化合物1，4，5对体外人类急性髓细胞性白血病细胞株HL-60的IG₅₀分别为27.78，32.22，26.19 μmol。结论：化合物1，3，5,8为首次从该属植物中分离得到。化合物1，4，5对体外人类急性髓细胞性白血病细胞株HL-60具有一定的生长抑制活性。

Abstract:Objective: To study the chemical constituents and antitumor activities of Euonymus alatus. Method: Compounds were isolated and purified repeatedly by silica gel, Sephadex LH-20 column chromatography, and their chemical structures were elucidated by their physicochemical properties and spectral data,such as ¹H-NMR, 13C-NMR and EI-MS. The trypan blue experiments in vitro were used to observe the antitumor activity of several compounds. Result: Ten compounds were obtained and identified as (+)-usnic acid (1), benzoic acid (2), 2-hydroxy-4-methoxy-3, 6-dimethyl benzoic acid (3), lupeol (4), 3β-hydroxy-30-norlupan-20-one (5), (2R, 3R)-3, 5, 7, 4′-tetrahydroxy flavanone (6), kaempferol (7), 5, 7, 4′-trihydroxy flavanone (8), quercetin (9) and daucosterol (10). Conclusion: Compounds 1, 2, 5 and 8 were isolated from this genus for the first time. The trypan blue experiments in vitro showed that compounds 1, 4 and 5 had growth inhibitory effects on HL-60 cells.

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