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期 刊 ：中国中药杂志 2008年 33卷 23期 页码：2838.

关键词：野菊花总黄酮；佐剂性关节炎；细胞凋亡；原位末端标记检测法；流式细胞术；

Keywords:total flavonoids of Chrysanthemum indicum (TFC), adjuvant arthritis, apoptosis, TUNEL(TdT-nediated dUTP nick end labeling), flow cytometer(FCM),

摘 要 ：目的：研究野菊花总黄酮(TFC)对佐剂性关节炎(AA)大鼠滑膜细胞的凋亡诱导作用，探讨TFC治疗AA的可能机制。方法：SD大鼠随机分成6组：正常组、模型组、TFC高、中、低剂量组和阳性药雷公藤多苷(TPT)组；SD大鼠右后足跖皮内注射弗氏完全佐剂复制AA模型；MTT法检测滑膜细胞的增殖；原位末端标记检测(TUNEL)法观察滑膜组织中滑膜细胞的凋亡改变；流式细胞仪检测体外培养滑膜细胞的凋亡变化。结果：AA大鼠滑膜细胞出现凋亡抑制，增殖过度的类瘤样细胞特征，TFC可剂量依赖性地降低AA大鼠滑膜细胞的炎性增殖；TFC(168，336 mg·kg-1)治疗组大鼠滑膜细胞的凋亡率较模型组显著升高。结论：TFC可抑制AA大鼠滑膜细胞的过度增殖，诱导滑膜细胞凋亡，这可能是其治疗AA的机制之一。

Abstract:Objective: To investigate the effect on the extract of total flavonoids of Chrysanthemum indicum (TFC) on adjuvant arthritis synovial cells. Method: SD rats were divided randomly into six groups including normal, model, TFC (84, 168, 336 mg·kg-1) and control drug Tripterygium glycosides (30 mg·kg-1) groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0.1 mL of the complete Freund’s adjuvant into the right hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MTT; The apoptosis rates of synovial cells were evaluated using TUNEL and FCM analysis. Result: TFC resulted in a dose-dependent way in inhibiting the proliferation of synovial and inducing the apoptosis of synovium and synoviocytes in vivo. Conclusion: TFC can inhibit proliferation and induce apoptosis in synovial cells, and exert therapeutical effect on rheumatoid arthritis.

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