Abstract:Objective: To study the chemical constituents in the aerial part of Reineckea carnea. Method: The compounds were isolated by extraction, silica gel, gel, and reversed-phase silica gel coloum chromatography, and high-performance liquid chromatography. The structures were identified by various spectroscopic methods including 1D and 2D NMR spectrum, MS, IR, etc. Result: Six compounds were isolated and identified as 1α, 3β-dihydroxy-5β-pregn-16-en-20-one-3-O-β-D-glucopyranoside (1), syringaresinol-β-D-glucoside (2), sophoraflavone B (3), stigmast-5, 22-dien-3-O-β-D-glucopyranoside (4), daucosterol (5), α-D-glucose (6). Conclusion: Compound 1 was a new compound, coumpounds 2-6 were obtained from the plant for the first time.