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期 刊 ：中国中药杂志 2007年 32卷 17期 页码：1792.

关键词：白黎芦醇；白介素1β；白介素6；肿瘤坏死因；脑缺血；

Keywords:resveratrol, interleukin-1β, interleukin-6, tumor necrosis factor-α, brain ischemia,

摘 要 ：目的：研究白黎芦醇对大鼠脑缺血/再灌注损伤后炎症的影响。方法：缺血前给予白黎芦醇10,20,40 mg·kg^-1,灌胃7 d,末次给药1 h后制备大鼠大脑中动脉阻断短暂局灶性缺血模型。脑缺血2 h再灌注24 h后,用TTC染色法、伊文思蓝法(EB)测定脑梗死范围及血脑屏障的损伤程度。分别用放免、酶免法测定大脑缺血区肿瘤坏死因子α(TNF-α)、髓过氧化物酶(MPO)的活性、血清白介素6,1β(IL-6,IL-1β)的含量。结果：白黎芦醇能缩小脑缺血/再灌注后脑梗死体积,减轻神经损伤症状,降低脑内TNF-α,MPO的活性及血清IL-6,IL-1β含量。结论：白黎芦醇可通过抑制脑缺血/再灌注过程中炎症介质释放,降低脑缺血/再灌注所致脑损伤。

Abstract:Objective: To investigate the protective effects of resveratrol on inflammatory process induced by focal cerebral ischemia-reperfusion in rats. Method: Rats were pretreated with resvreratrol at the dose of 10, 20, 40 mg·kg^{-1 for 7 days and then subjected to cerebral ischemia/reperfusion induced by a middle cerebral artery occlusion (MCAO). The infarct volume and the neurological deficit were determined by the method of TTC (2, 3, 5-triphenylterazolium chloride) staining and Longa‘s score. The permeability of blood-brain barrier (BBB) was evaluated by measurement of the evans blue (EB) content in the brain with spectrophotometer. The content of interleukin-1β, interleukin-6 (IL-6, IL-1β) in serum and tumor necrosis factor-α (TNF-α), myeloperoxidase (MPO) in brain were determined by radio-immunoassay and ELISA assay. Result: Resveratrol reduced infarct volume, ameliorated the neurological deficit and the permeability of BBB, the content of IL-6, IL-1β in serum and TNF-α, MPO activity in brain tissue also were significantly decreased. Conclusion: These results showed that resveratrol had protective effects on cerebral injury by inhibiting the releasing of the inflammatory mediators after ischemia/reperfusion injury.}

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