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期 刊 ：中国中药杂志 2007年 32卷 09期 页码：779.

关键词：黄芪；毛蕊异黄酮葡萄糖苷；芒柄花素；高效液相色谱；含量测定；

Keywords:Radix Astragali, calycosin-7-O-β-D-glycoside, formononetin, HPLC, quantitative analysis,

摘 要 ：目的：建立黄芪药材中毛蕊异黄酮葡萄糖苷和芒柄花素的含量测定方法,并对不同来源的药材进行分析,为黄芪质量标准的制定提供依据。方法：采用HPLC-DAD法建立黄芪药材中毛蕊异黄酮葡萄糖苷和芒柄花素的含量测定方法,通过对来源于我国8个省59批黄芪药材的分析,阐明产地、生长方式、生长年限等对黄芪中异黄酮类成分的影响。结果：不同产地、生长方式及生长年限的黄芪药材,其毛蕊异黄酮葡萄糖苷和芒柄花素的含量差异较大。从产地分析,以山西、内蒙古和陕西等道地产地含量较高；从生长方式分析,以半野生含量较高；从生长年限分析,年限越短,含量越高。结论：本研究建立的毛蕊异黄酮葡萄糖苷和芒柄花素的含量测定方法简便、准确、重现性好,可用于黄芪药材中异黄酮类成分的质量控制。

Abstract:Objective: To establish the quantitative methods for calycosin glycoside and formononetin in Radix Astragali, and the samples from different sources were analyzed, in order to supply the basis for the quality control of Radix Astragali. Method: The content of calycosin glycoside and formononetin in 59 samples of Radix Astragali from eight with different provinces was analyzed by HPLC-DAD. Result: The contents of calycosin glycoside and formononetin in Radix Astragali from different sources, with different cultivating method or in different ages differed markedly, and the results showed that the quality of samples from Shannxi, Innermongolia and Shanxi were better than other sources, and the semi-wild samples were better than other cultiving samples, moreover the shorter age, the better quality. Conclusion: This simple, accurate and reproducible method could use to determine the contents of isoflavanoids in Radix Astragali.

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