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期 刊 ：中国中药杂志 2003年 28卷 07期 页码：603.

关键词：黄芪；毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷；高效液相色谱法；含量测定；

Keywords:Radix Astragali, calycosin-7-O-β-D-lucopyranoside, HPLC, determination,

摘 要 ：目的:建立黄芪中毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷含量测定的高效液相色谱法,并测定10批不同来源黄芪商品药材中该成分的含量。方法:色谱柱为Polaris C₁₈柱(4.6mm×250mm,5μm),流动相为甲醇-水(30:70),流速为1.0 mL·min^-1,柱温为25℃,检测波长为254 nm。结果:毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷在0.010 6～2.12μg呈良好的线性关系,回归方程为Y=3 035.97X-14.85(r=0.9999),加样回收率平均为95.8％(n=5,RSD=1.3％)。结论:本法简便、快速、灵敏,重现性好。所测10批不同来源黄芪商品药材中毛蕊异黄酮-7-O-β-D-葡萄吡喃糖苷含量差异较大。

Abstract:Objective: TO establish a RP-HPLC method for the determination of calycosin-7-O-β-D-glucopyranoside in Radix Astragali, and to analyse the calycosin-7-O-β-D-glucopyranoside content of ten samples of Radix Astragali, collected from different regions. Method: A Polaris C₁₈(250 mm×4.6 mm, 5 μm)column was used and a mixture of methanol-water (30:70) was used as the mobile phase at a flow rate of 1. 0 mL·min^-1. The column temperature was 25℃and the UV detection wavelength was 254 nm. Result: The calibration curve was in good linearity over the range of 0.0106-2.12 μug with the regression equation Y= 3035. 97X-14.85(r =0.9999). The average recovery was 95.8 % (n = 5, RSD =1.3%). Conclusion: The method is simple, quick, sensitive and reproducible. In all of the samples, the calycosin-7-O-β-D-glucopyranoside contents differ markedly.

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