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期 刊 ：中国中药杂志 2005年 30卷 11期 页码：861.

关键词：脂多糖；左旋四氢巴马汀；黏附分子；人脐静脉内皮细胞；

Keywords:lipopolysaccharides, l-tetrahydropalmatine, adhesion molecules, human umbilical vein endothelium cell,

摘 要 ：目的:观察左旋四氢巴马汀(l-THP)对脂多糖(lipopolysaccharides,LPS)诱导人脐静脉内皮细胞(HU-VEC)细胞间黏附分子-1(ICAM-1)和E选择素(E-selectin)表达的影响,探讨l-THP的抗炎作用。方法:分离纯化人脐静脉内皮细胞,与LPS或LPS加不同浓度l-THP共育,用流式细胞仪检测细胞表面黏附分子表达的变化。结果:l-THP(250mg·L^-1)显著降低LPS诱导的ICAM-1和E-selectin表达(P<0.05)。l-THP(50mg·L^-1)抑制E-selectin表达(P<0.05),然而对ICAM-1的表达无明显影响(P>0.05)。l-THP(10mg·L^-1)对2种黏附分子表达均无明显影响(P>0.05)。50mg·L^-1地塞米松(DXM)完全抑制LPS诱导上述2种黏附分子的表达(P<0.05)。结论:左旋四氢巴马汀能减少LPS诱导的ICAM-1和E-selectin表达,提示l-THP可开发成炎症治疗药物。

Abstract:Objective: To investigate the effect of l-tetrahydropalmatine (l-THP) on adhesion molecule expression induced by LPS in human umbilical vein endothelium cell (HUVEC). Method: HUVEC were obtained from the human umbilical cord vein and treated by LPS or LPS plus different concentration of l-THP. Expression of ICAM-1 and E-selectin was assayed by flow cytometer. Result: l-THP(250 mg·L^-1)markedly attenuated ICAM-1 and E-selectin expression induced by LPS(P<0.05) . l-THP(50 mg·L^-1) inhibited E-selectin expression (P<0.05) but had no effect on ICAM-1(P> 0.05). l-THP(10 mg·L^-1) had no effect on expression of both adhesion molecules. DXM(50 mg·L^-1) completely inhibited both responses induced by LPS (P<0.05). Conclusion: l-THP depresses expression of ICAM-1 and E-selectin induced by LPS, which suggests that l-THP represent a promising candidate to be developed into therapies for the treatment of inflammation.

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