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期 刊 ：中国中药杂志 2005年 30卷 11期 页码：851.

关键词：鹿茸；鹿血清白蛋白；分离纯化；UMR-106细胞；胰岛素样生长因子Ⅰ；

Keywords:pilose ander, deer serum albumin, UMR-106 cells, IGF-Ⅰ,

摘 要 ：目的:通过鹿血清白蛋白对大鼠成骨样细胞系UMR-106增殖活性和促胰岛素样生长因子Ⅰ(IGF-Ⅰ)分泌水平的研究,为阐明鹿茸强筋健骨的作用机理及对骨质疏松的防治提供研究线索。方法:运用SephacrylS-200HR凝胶色谱、POROS20QE阴离子交换色谱和TSKG3000SW高效液相色谱等分离手段,从冷冻干燥鹿茸粉粗提液中分离纯化得到鹿血清白蛋白。并用MTT法检测其对UMR-106细胞的增殖活性;用平衡饱和竞争放射免疫分析法(RIA)测定其促IGF-Ⅰ分泌水平。结果:经分离纯化得到的鹿血清白蛋白分子质量为56.3kDa,在蛋白含量为0.149μg·mL^-1时,其促大鼠成骨样细胞增殖率为241.03%,IGF-Ⅰ分泌量为66.89ng·mL^-1。结论:鹿茸中的鹿血清白蛋白在14.9ng·mL^-1-14.90μg·mL^-1具有显著的促大鼠成骨样细胞增殖和IGF-Ⅰ分泌等活性。

Abstract:Objective: The activity of deer serum albumin on proliferation of rat osteogenic-like cells UMR-106 and the IGF-Ⅰ secretion were investigated in order to elucidate pilose antler‘ s bone-strengthening mechanism. Method: Deer serum albumin was isolated from freeze-dry pilose antler powder extract. The methods were Sephacryl S-200HR gel filtration, POROS 20QE ion-exchange and TSK G3000SW chromatographies. The effect of deer serum albumin on proliferatio of UMR-106 cells was assaied by MTT, and the secretion of IGF-Ⅰ of UMR-106 cells was assaied by RIA. Result: Deer serum albumin, with the molecular weight of 56.3 kDa, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-Ⅰ secretion. When concentration of deer serum albumin reached 0.149 μg·mL^-1, UMR-106 cell proliferation rate was 241.03% and IGF-Ⅰ secretion was 66.89 ng·mL^-1. Conclusion: The concentration of deer serum albumin, from 14.9 ng·mL^-1 to 14.90 μg·mL^-1, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-Ⅰ secretion.

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