Abstract:The establishment of a quantitative determination method by HPLC for adenosine and 3‘-deoxyadenosine in Cordyceps militaris is reported.The sample was extracted with water under ultrasonic management for 2 hours.Chromatographic conditions:YWG C1810μm 5mm×200mm;mobile phase consisted of NaH2PO4NaOH buffer solution (pH 6.8);UV detection at 260nm.The recoveries of adenosine and 3‘ deoxyadenosine were 95.8%±0.64% and 100.6%±0.65%(n=5) respectively.