Abstract:Objective: To establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis. Method: The column used was a Kromasil C18(4.6 mm ×250 mm) packed with a 5μm stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW=119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1 000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0. 8 mL per minute; the column was maintained at 40℃; the DAD detector was set at 210 run. Results: The liner range was 0.251 -10.04μg (r=1.0000). An average recovery of 98.1 % (n=6) was obtained with a RSD of 1.0% . Conclusion: The method is simple, accurate and suitable for the qualify control.