Abstract:A polysaccharide——Chai-Ⅲ-5311 had been isolated from hot water extract of Radix Bupleuri through ultrafiltration and dialysis. Chai-Ⅲ-5311 was homogeneous with an average molecular weight of about 8000. Acid hydrolysis has proved that Chai-Ⅲ-5311 is composed of galacturonic acid, galactose, glucose, arabinose, xylose, rihose, rhamnose and an unknown component.