Abstract:1-(2-pyridylazo)-2-naphthol (PAN) and Emulsifier OP hava been used in the colorimetric determination of zinc in Gynostemma pentaphyllum. The results are in agreement with those of atomicabsorption spectrophotometry. The method is simple, precise and rapid. The recovery is 97.8~103.7% and coefficient of veriation is 1.4~4.6%.