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作 者 ：丁燕飞,姚瑶,陶昱斐,冯秀珍

期 刊 ：中草药 2008年

关键词：槲皮素；脂质体；薄膜蒸发-高压均质法；鱼精蛋白沉淀法；

Keywords:quercetin, liposomes, film dispersion-homogenizing method, protamine sedimentation method,

摘 要 ：目的制备槲皮素脂质体,并测定其包封率。方法采用薄膜蒸发一高压均质法制备槲皮素脂质体,通过正交设计优化处方工艺,以鱼精蛋白沉淀法分离脂质体与游离药物,测定药物的量.并计算包封率。结果优化的最佳处方工艺:胆固醇与磷脂比为1:3;药物与载体比为1:40;103.4 MPa压力下均质3次。最佳条件下制得的脂质体平均包封率达92.1%。结论薄膜蒸发-高压均质法适合于制备槲皮素脂质体。鱼精蛋白沉淀法测定包封率操作简单,准确。

Abstract:Objective To prepare quercetin liposomes and establish a method for determination of its entrapment efficiency.MethodsMethods The film dispersion-homogenizing method was used to prepare quercetin liposomes.The formulation was optimized on the basis of orthogonal design and its entrapment efficiency was performed by the protamine sedimentation method.Results The optimal conditions were found to be cholesterol-egg phospholipid=1:3,quercetin-vehicle = 1:40,homogenization pressure 103.4 MPa for three times.The average entrapment efficiency of the optimized nano-liposomes was 92.1%.Conclusion The film dispersion-homogenizing method could be used to prepare quercetin liposomes.The protamine sedimentation method is convenient,accurate,and suitable for the determination of the entrapment effi- ciency of quercetin liposomes.

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