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作 者 ：刘建新,胡浩斌,王 鑫.

期 刊 ：中国生态农业学报 2008年 16卷 5期 页码：1173-1177

关键词：骆驼蓬生物碱；黄瓜；种子萌发；外源Ca2+赤霉素；缓解效应；加成作用；

Keywords:Peganum harmala alkaloid, Cucumber, Seed germination, Exogenous Ca2+, Gibberellin, Ameliorative effect, Synergistical effect,

摘 要 ：采用室内水培方法，研究了外源Ca²⁺和GA₃对骆驼蓬生物碱抑制下黄瓜种子萌发的影响。结果表明，Ca²⁺和GA₃单一及复合处理可促进骆驼蓬生物碱处理的黄瓜种子的萌发，使黄瓜萌发率、发芽指数和活力指数提高，萌发种子的α-淀粉酶、蛋白酶、脂肪酶和异柠檬酸裂解酶活性及赤霉素(GA)和钙调素(CaM)含量增加，烟酰胺腺嘌呤二核苷酸激酶(NADK)和Ca²⁺—ATP酶活性增强，脱落酸(ABA)含量降低；且Ca²⁺和GA₃复合处理对GA含量的提高和ABA含量的降低与Ca²⁺单一处理比较呈显著的加成作用。表明外源Ca²⁺和GA₃对骆驼蓬生物碱抑制黄瓜种子的萌发具有缓解效应。

Abstract:A hydroponics experiment was conducted to study the effect of exogenous Ca²⁺ and GA₃ on cucumber germination inhibited by Peganum harmala alkaloid. The results indicate that Ca²⁺ and GA₃, single or combined application treatments, promote cucumber seed germination treated with P.harmala alkaloid, increase germination rate, germination vigor and index; as well as α-amylase, protease, lipase, isocitratelyase, nicotinamide adenine dinucleotide kinase (NADK) and Ca²⁺—ATPase activities; and GA and CaM content in cucumber seeds; but decrease abscisic acid (ABA) content. The synergistic effects of combined Ca²⁺ and GA3 on increasing GA and decreasing ABA are oberseved compared with Ca²⁺ treatment. It shows that exogenous Ca²⁺ and GA₃ can alleviate P. harmala alkaloid inhibition in cucumber germination.

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