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作 者 ：孙红艳,林瑞余,叶陈英,于翠平,黄 磊,陈冬梅,林文雄.

期 刊 ：中国生态农业学报 2008年 16卷 4期 页码：894-899

关键词：小麦；化感作用；种质；筛选；土壤-琼脂三明治法；

Keywords:Triticum aestivum L., Allelopathy, Germplasm, Screening, Agar-Soil-Sandwich Method,

摘 要 ：以国内外90份不同小麦为材料,采用土壤-琼脂三明治法和田间杂草调查法,对不同小麦种质资源进行化感潜力评价，筛选出具有较强化感潜力的小麦品种。土壤-琼脂三明治法测试结果表明，不同小麦品种的化感潜力存在明显差异，对莴苣根长抑制率IR在10.1%和69.1%之间；筛选出“115/青海麦”、“92L89”、“百泉3199”、“81-214”和“92H31”5个强化感潜力的小麦品种，其对莴苣根长抑制率依次为69.1%、68.5%、68.7%、69.1%和65.2%，筛选出“抗10103（80）”和“A246”2个弱化感潜力的小麦品种，抑制率IR依次为10.1%和12.2%；田间杂草调查评价和土壤-琼脂三明治法表现出相似趋势，检测结果也得到了琼脂迟播共培法的佐证，表明采用土壤-琼脂三明治法筛选化感小麦种质资源是可行的。这为小麦化感作用研究提供了重要的种质资源，也为化感小麦种质资源的筛选提供方法借鉴。

Abstract:By the bioassay of Agar-Soil-Sandwich Method (ASSM) and field investigation, 90 wheat cultivars (originating from China and abroad) were used to evaluate the allelopathic potential and screene several stronger allelopathic wheat germplasm. 5 wheat cultivars “115/Qinghaimai”, “92L89”, “Baiquan3199”, “81-214” and “92H31”, tested for strong allelopathic potential, were screened by ASSM method. The results show that different wheat cultivars have significantly different allelopathic potential, with inhibition rate (IR) of 10.1%～69.1% against lettuce (Lactuca sativa L.). Lettuce root IR in the 5 selected wheat cultivars is 69.1%, 68.5%, 68.7%, 69.1% and 65.2% respectively. In addition, 2 weak allelopathic potential wheat cultivars, “Kang 10103(80)” and “A246”, with IR of 10.1% and 12.2%, are found among the tested germplasms. The wheat germplasm, following the stronger allelopathic potential test by ASSM bioassay also shows strong allelopathic potential in the field, and confirmed by relay seeding in the agar method. This suggests that ASSM is a feasible method for screening allelopathic wheat. Consequently, the screened wheat cultivars are valuable for allelopathic wheat breeding, which can serve as an important germplasm. This can equally be a reference method in screening allelopathic potential in wheat germplasm.

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