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A Method for Constructing Unmarked Deletion Mutants of Pseudomonas plecoglossicida NyZ12 Polycomb-group histone methyltransferase CLF is required for proper somatic recombination in Arabidopsis The Effect on Phosphatidylserine Biosynthesis of Gene sdaA，sdaB，pgpB in Escherichia coli Expression of Fructose Valine Oxidase in Sf9 Cells Foreign Protein Expression in Chloroplast Transformation of Phaeodactylum tricornutum The Research of Genome Editing Integrative Expression of Stichopus japonicus Lysozyme Gene in Bacillus subtilis